广西师范大学学报（自然科学版）2018，Vol.36Issue(1)：99-104,6.DOI:10.16088/j.issn.1001-6600.2018.01.014
MEKC测定麻花艽中齐墩果酸和熊果酸含量
Determination of Oleanic Acid and Uosolic Acid in Gentiana straminea Maxim.by MEKC
摘要
Abstract
A micellar electrokinetic chromatography (MEKC)method was established for determining the contents of oleanic acid and ursolic acid in Gentiana straminea Maxim..The separation was performed on an uncoated fused silica capillary (64.5 cm×50 μm,56 cm of effective length)with a voltage of 15 kV.A 20％ acetonitrile solution containing 70 mmol/L borax and 35 mmol/L SDS(pH=9. 85)was used as the buffer solution.The sample was injected at a pressure of 5 kPa×5 s and detected at a wavelength of 210 nm.The results showed a good linearity between the concentration and peak area (r>0.9992).The RSD of two active ingredients were 4.21％ and 3.87％.This MEKC method has the advantages of simple,accurate and reproducible.It could be used for the quality evaluation and control for Gentiana straminea Maxim..关键词
胶束毛细管电泳(MEKC)/麻花艽/齐墩果酸/熊果酸/含量Key words
micellar electrokinetic chromatography(MEKC)/Gentiana straminea Maxim./oleanic acid/分类
化学化工引用本文复制引用
张军文,李成思,卢永昌,史生辉..MEKC测定麻花艽中齐墩果酸和熊果酸含量[J].广西师范大学学报（自然科学版）,2018,36(1):99-104,6.基金项目
国家自然科学基金(81160554) （81160554）
