核农学报2017，Vol.31Issue(12)：2340-2349,10.DOI:10.11869/j.issn.100-8551.2017.12.2340
中国东北地区绿豆种质资源遗传多样性的SSR分析
Genetic Diversity Analysis of Mungbean Germplasm Resources From Northeast China Region With SSR Makers
摘要
Abstract
To analysis the genetic diversity of mungbean germplasm resources from Northeast China region,mungbean vriaties of 156 from different areas of Northeast China were analyzed by using 15 SSR markers.A total of 71 alleles were identified with an average of 4.7 alleles per locus,and the polymorphic information content(PIC) varied from 0.067 to 0.754,with an average of 0.401.The genetic similarity coefficient of SSR markers among 74 mungbean varieties ranged from 0.116 to 1.000,with an average of 0.542,and the genetic similarity coefficient of 73.69％ tested materials ranged from 0.50 to 1.00.Furthermore,the UPGMA(unweighted pair group method with arithmetic mean) clustering analysis classified the 156 mungbean varieties into five groups at the genethc strnirlarity value of 0.478 and varieties with the same geographic origin are mostly clustered together.The genetic relationship among mungbean varieties from Northeast China was close,and the genetic background comparability of the mungbean varieties was high and the diversity was low.Therefore,new products and germplasm resources introduction should be strengthened to create mungbean breeding materials and enrich genetic diversity of mungbean germplasm resources in Northeast China.The results of this study provide technical support and theoretical basis for clarifying the relationship of mungbean varieties in northeast China and the selection of new products.关键词
绿豆/遗传多样性/SSR荧光标记/东北地区Key words
mungbean/genetic diversity analysis/fluorescent labeled SSR markers/Northeast China region引用本文复制引用
赵雅楠,王颖,张东杰,王丽侠,佐兆杭..中国东北地区绿豆种质资源遗传多样性的SSR分析[J].核农学报,2017,31(12):2340-2349,10.基金项目
黑龙江省应用技术与开发重大项目(GA14B104),大庆科技局创新项目(sjh-2013-65),黑龙江省研究生创新科研项目资金(YJSCX2017-Y50) （GA14B104）
