湖南中医药大学学报2018，Vol.38Issue(1)：21-26,6.DOI:10.3969/j.issn.1674-070X.2018.01.006
三七总皂苷预处理对大鼠肾缺血再灌注损伤的保护作用及机制研究
Protective Effect and Mechanism Research of Panax Notoginseng Saponins Pretreatment on Renal Ischemia Reperfusion Injury of Rats
摘要
Abstract
Objective To investigate the protective effect and its mechanism of panax notoginseng saponins (PNS) on re-nal ischemia reperfusion injury (RIRI) in rats. Methods 40 male SD rats were randomly divided into the sham-opera-tion group, model group, low dose, medium dose, high dose [40, 80, 160 mg/(kg·d)] PNS groups. All rats were administered for 5 d, respectively. After ending treatment for 1 h, the RIRI model was established. After reperfusion for 3 h, the levels of urine nitrogen (BUN), secrumcreatinine (SCr), the content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in serum were measured. The pathological changes of kidney tissue were investigated by optical microscopy and hematoxylin-eosin(HE) staining techniques. The levels of intercellular cell adhesion molecule-1(ICAM-1) and nuclear fac-tor kappa B p65 (NF-κB p65) in renal tissue were evaluated by immunohistochemical staining. Results Compared with sham-operation group, the levels of serum Cr, BUN, MDA and renal tissue ICAM-1, NF-κB p65 increased in model group, the in-jury of renal tubule was obvious, SOD decreased, the differences were statistically significant (P <0.01). Compared with model group, the levels of serum Cr, BUN, MDA and renal tissue ICAM-1, NF-κB p65 in PNS groups decreased, pathological injury of kidney tissue relieved, while SOD increased, the differences were statistically significant (P<0.01). The indicators between PNS groups were not statistically significant (P>0.05). Conclusion PNS can improve the RIRI in rats through decreasing oxidative stress and inflammation.关键词
三七总皂苷/肾缺血再灌注损伤/核因子-κBp65/细胞间黏附因子-1Key words
panaxnoto ginseng saponins/renal ischemia reperfusion injury/nuclear factor kappa B p65/intercellular cell adhesion molecule-1分类
医药卫生引用本文复制引用
孙雯怡,罗旺辉,周铖,陈珍妮,李洋洋,罗琳,唐群,张煕..三七总皂苷预处理对大鼠肾缺血再灌注损伤的保护作用及机制研究[J].湖南中医药大学学报,2018,38(1):21-26,6.基金项目
国家自然科学基金青年项目(81503442、81603470) （81503442、81603470）
中西医结合基础湖南省"十二五"重点学科开放基金资助项目(ZXJCXK201503) （ZXJCXK201503）
湖南省大学生研究性学习和创新性实验计划项目(2015-216) （2015-216）
湖南省教育厅优秀青年项目(17B197、16B196). （17B197、16B196）
