湖南中医药大学学报2018，Vol.38Issue(1)：27-30,4.DOI:10.3969/j.issn.1674-070X.2018.01.007
芦笋提取物抗氧化和抑制3T3-L1前脂肪细胞分化活性研究
Study of the Activities of Antioxidant and Inhibiting Differentiation of 3T3-L1 Preadipocytes of the Extract from Phragmites australis (Cav.) Trin. ex Steud
摘要
Abstract
Objective To study the activities of antioxidant and inhibiting differentiation of 3T3-L1 preadipocytes of Lusun [Phragmites australis (Cav.) Trin. ex Steud] extract. Methods The Lusun exatract was prepared by using ethanol re-flux extraction and acid and alkali treatment. The content of phenolic acid of the extract was mearsured by UV spectropho-tometry. The activity of free radical scavenging of extract was measured by using DPPH method. The effects of the extract on the survival rate and differentiation rate of 3T3-L1 preadipocytes were detected by CCK8 and oil red O staining. Results The content of phenolic acid in the Lusun extract was 0.5308 mg/mg calculated by gallic acid. The activity of scavenging free radicals of Lusun extract has positive correlation with dose, the DPPH removal rate was 89.68％ when the concentration reached 2 mg/mL. The Lusun extract at the concentration of 10-200 μg/mL had no significant effect on the survival rate of 3T3-L1 cells, and had obvious inhibiting effect on the differentiation of 3T3-L1 cells in a dose-dependent manner. Conclu-sion The Lusun extract has significant scavenging free radical activity and have remarkable inhibition effect on 3T3-L1 cells proliferation, and the activities increase with the dose.关键词
芦笋/酚酸/抗氧化/3T3-L1前脂肪细胞Key words
Phragmites australis (Cav.) Trin. ex Steud/phenolic acid/antioxidation/3T3-L1 preadipocytes分类
医药卫生引用本文复制引用
张雨林,谭洋,詹济华,满兴战,李雪,裴刚..芦笋提取物抗氧化和抑制3T3-L1前脂肪细胞分化活性研究[J].湖南中医药大学学报,2018,38(1):27-30,4.基金项目
湖南省教育厅科学研究项目(16K064) （16K064）
长沙市科技局资助项目(kq1602015) （kq1602015）
湖南省芦笋研究开发中心芦笋产业联合研究开发基金. （）
