湖南中医药大学学报2018，Vol.38Issue(1)：55-58,4.DOI:10.3969/j.issn.1674-070X.2017.01.015
针刺大椎、人中、百会穴对脑缺血再灌注损伤大鼠脑线粒体超微结构的影响
Effects of Acupuncture Dazhui, Renzhong, Baihui Points on Ultrastructure of Cerebral Mitochondria in Rats with Cerebral Ischemia Reperfusion
摘要
Abstract
Objective To observe the effect of acupuncture Dazhui, Renzhong, Baihui on the ultrastructure of cerebral mitochondria after cerebral ischemia reperfusion in rats and explore its mechanisms. Methods Ten rats were randomly selected from 40 rats as sham -operation group, the remaining 30 rats after modeling were randomly divided into 3 groups: model group, acupuncture control point group and acupuncture point group, 10 rats in each group. After succesfully modeling of rats, the model group and the sham operation group were only bundled without acupuncture, the acupuncture point group was acupunctured of Dazhui, Renz hong, Baihui point, the acupuncture control point group was acupunctured of the point left side 0.3 cm, once every 12 hours, and killed the rats after 6 times of treatment. The hippocampus mitochondria ultrastructure was observed by electron microscope. Results After treatment, the neurological deficit scores in different groups besides sham-operation group decreased (P<0.05); compared with the model group, the decrease in acupuncture control point g roup and acupuncture point group was obvious (P<0.05). Electron microscope showed that the structure of mitochondrial was normal in the sham-operation group, the mitochondria in the model group showed apparent swelling, disrupted cristae and vacuoles were obvious, cristae broken and slightly vacuolization was observed in the acupuncture control group; swelling, cristae broken, and the vacuolization of the mitochondria in the acupuncture point group was not obvious. Conclusion Acupuncture could relieve the injury of cerebral ischemia reperfusion, its mechanism may be related to the alteration of ultrastructural damage of mitochondria in ischemia-reperfusion injury.关键词
针刺/线粒体超微结构/脑缺血再灌注损伤Key words
acupuncture/mitochondria ultrastructure/cerebral ischemia reperfusion分类
医药卫生引用本文复制引用
贺平,颜虹,蒋素容,武姿含,陈楚淘,田浩梅..针刺大椎、人中、百会穴对脑缺血再灌注损伤大鼠脑线粒体超微结构的影响[J].湖南中医药大学学报,2018,38(1):55-58,4.基金项目
湖南自然科学基金(81303051) （81303051）
湖南省自然科学基金(2016JJ3131) （2016JJ3131）
中医内科学省部共建教育部重点实验室开放课题. （）
