湖南中医药大学学报2018，Vol.38Issue(1)：59-64,6.DOI:10.3969/j.issn.1674-070X.2018.01.016
合募配穴针刺对肠易激综合征模型大鼠脊髓背根神经节PAR-2、TRVP1及相关致敏细胞因子表达的影响
Effects of He-Mu Adjunct Acupuncture Points on the Expression of PAR-2, TRVP1 and Related Sensitized Cytokines in Dorsal Root Ganglia of IBS Model Rats
摘要
Abstract
Objective To observe the effect of He-Mu adjunct acupuncture points on the protease-activated receptor-2 (PAR-2), transient receptor potential vanilloid 1(TRPV1) and calcitonin gene related to peptide (CGRP) of dorsal root ganglion (DRG) of the rat spinal cord in rats with irritable bowel syndrome (IBS), and to explore the possible mechanism of raised acupuncture on visceral hypersensitivity. Methods The male SD IBS model rats were built by using the chronic stress bind-ing method. The model rats were randomly divided into blank group, model group, He-Mu acupuncture group and drug group. After successfully building the model, the He-Mu acupuncture group was acupunctured at Xiahe point and Tianshu point of the colon. The drug group was gavaged with pinaverium bromide. The visceral sensitivity of the rats was evaluated by abdom-inal back reflex. The expression of PAR-2, TRVP1, SP and CGRP in dorsal root ganglion of spinal cord was detected by im-munohistochemistry. Results Before treatment, there were significant differences in the AWR scores between the acupunc-ture group and the model group, compared with the blank group (P<0.01); after treatment, there was significant difference in the AWR scores between the acupuncture group and the model group (P<0.01). Acupuncture in treatment of visceral hypersensitivity of IBS is effective. Compared with the blank group, PAR-2, SP, TRVP1 and CGRP increased significantly in the model group (P <0.01). PAR -2, SP, TRVP1 and CGRP significantly decreased in acupuncture group and drug group, compared with model group (P<0.01). Conclusion He-Mu adjunct acupuncture points can reduce the expression of SP and CGRP in the DRG through PAR-2 and TRVP1, to reduce the visceral hypersensitivity of IBS rats.关键词
肠易激综合征/合募配穴针刺/蛋白酶活化受体-2/P物质/瞬时受体电位香草酸亚型1/降钙素相关肽Key words
irritable bowel syndrome/He-Mu adjunct acupuncture points/protease-activated receptor-2/substance p/transient receptor potential vanilloid 1/calcitonin gene related to peptide分类
医药卫生引用本文复制引用
郭奎奎,邓多喜,张泓,黎帅,林英惠,谭洁..合募配穴针刺对肠易激综合征模型大鼠脊髓背根神经节PAR-2、TRVP1及相关致敏细胞因子表达的影响[J].湖南中医药大学学报,2018,38(1):59-64,6.基金项目
湖南省中医药科研计划重点资助项目(201525) （201525）
湖南省教育厅科学研究项目优秀青年项目(16B197). （16B197）
