吉林农业大学学报2017，Vol.39Issue(6)：640-647,8.DOI:10.13327/j.jjlau.2017.3739
农杆菌介导的玉米幼胚遗传转化体系的建立
Establishment of Genetic Transformation System of Maize Immature Embryos Mediated by Agrobacterium tumefaciens
摘要
Abstract
With maize immature embryos as receptor materials,the plant expression vector pCAM-BIA1300-35S-GUS-Nos was introduced into maize immature embryos by Agrobacterium-mediated method.With GUS transient expression efficiency as indicator,the factors,such as,genotype,immature embryo size,infection liquid concentration and infection time,were studied by single factor test and the L9(34) orthogonal experiment to explore their effects on genetic transformation rate.The results show that:The effect rank of these factors was genotype＞embryo size＞bacterial concentration ＞infection time;The optimal infection rate was the genotype H99,the size of immature embryos was 1.1-2.0 mm,OD600 =0.6,the time of infection was 30 min,and the transient expression rate of GUS gene was (34.44± 1.11) ％.关键词
玉米幼胚/农杆菌介导法/遗传转化率/体系优化Key words
maize immature embryo/Agrobacterium-mediated method/genetic transformation rate/system optimization分类
农业科技引用本文复制引用
魏晓禹,邵诗迪,孙苏,金栋梁,刘智博,焦鹏,关淑艳..农杆菌介导的玉米幼胚遗传转化体系的建立[J].吉林农业大学学报,2017,39(6):640-647,8.基金项目
吉林省科技攻关项目(20170204005NY),吉林省粮食生产发展专项资金项目(2015001),吉林省教育厅“十三五”科学研究规划项目(JJKH20170308KJ),吉林农业大学科研启动基金项目(201315) （20170204005NY）
