吉林农业大学学报2017，Vol.39Issue(6)：655-664,10.DOI:10.13327/j.jjlau.2017.3899
甲基磺酸乙酯诱导的大豆M1候选多性状共变体重要农艺性状分析
Agronomic Traits of M1 Multi-Trait Mutants by EMS Mutagenesis in Soybean
摘要
Abstract
In large-scale induction of mutation,screening of hereditable mutants at subsequent generations depends on the identification of candidate mutants from M1 population induced by EMS,and screening of multi-trait mutants is helpful to polygene aggregation breeding.In the experiment,with an early-maturity soybean cultivar "Yannong 11" as material,mutations were induced by 0.4％ EMS treatment of soybean seeds,and by the phenotypic evaluation of the whole plant performance,candidate mutants were identified and screened.The results showed that 18 types of mutations occurred;mutation rate for each trait varied from 0.1％ to 0.2％,and the number of traits mutated of each composite trait mutant was from 2 to 5.In addition,4 negative transgressive multi-trait mutants were screened;their individual plant pod number (IPPN),grain number (IPGN) and grain weight (IPGW) were only 1/2 to 1/3 of the wild type.Five positive transgressive multi-trait mutants were screened;their IPPN,IPGN and IPGW were 1.9 to 3.0 times as much as the wild type.Among these,candidate mutant M1-31 was characterized by resistance,super pod number,super grain number and super four-grain pod number,and its IPGN,IPGW and four-grain pod number were 2.1,1.9 and 3.7 times as much as the wild type.In mutant population,one-grain pod and two-grain pod were significantly correlated (P＜0.01),so were two-grain pod and three-grain pod.One-grain pod was positively correlated with three-grain pod,and one-grain pod was negatively correlated with four-grain pod,whereas two-grain pod and three-grain pod were positively correlated with four-grain pod,but with low correlation coefficients (r ＜ 0.2,P ＞ 0.05).These data are helpful to high throughput screening of mutants from subsequent generation progeny populations derived from M1 candidate mutants,which will lay a foundation for constructing high-capacity mutant pools,and exploring and analyzing functional genes in soybean.关键词
大豆/甲基磺酸乙酯/多性状共变体/候选突变体/农艺性状Key words
soybean/EMS/multi-trait mutation/candidate mutant/agronomic trait分类
农业科技引用本文复制引用
李永一,张化瀛,孙云轩,黄初女,朴世领,郑大浩..甲基磺酸乙酯诱导的大豆M1候选多性状共变体重要农艺性状分析[J].吉林农业大学学报,2017,39(6):655-664,10.基金项目
延边大学校科研启动基金项目(2011800-950118010),吉林省高等教育专项资金项目(012013002),国家自然科学基金项目(31660395) （2011800-950118010）
