吉林农业大学学报2017，Vol.39Issue(6)：695-702,708,9.DOI:10.13327/j.jjlau.2017.3585
人参法尼基焦磷酸合成酶基因的表达及其与皂苷含量的关系
Correlation between Panax ginseng Farnesyl Diphosphate Synthase Gene Expression and Ginsenoside Content
摘要
Abstract
Using five-year ginseng root tissue as experimental materials,ginseng farnesyl pyrophosphate synthase (FPS) gene was cloned.Protein characteristics were analyzed by bioinformatics,and phylogenetic tree of FPS was constructed using MEGA5.1.Tissue expression in different growth stages was analyzed using RT-PCR technology.Content of 8 kinds of ginsenosides in root was determined by HPLC and correlation analysis was conducted.1 134 bp FPS sequence was obtained,which has a complete open reading frame,encoding an unstable protein with 342 amino acids.FPS encoding protein has isoelectric point of 5.83 and a calculated relative molecular weight about 39 567,without transmembrane regions and signal peptide.In the secondary structure,the main alpha helix accounted for 58.19％.The homologous analysis indicated nucleotide sequence was 99％ homologous with Panax quinquefolius D S (ADJ68004) and Panax notoginseng D S (AGS79228).Real-time quantitative PCR showed that FPS gene was expressed in all growth stages.Expression level of Panax ginseng FPS was the highest in leaf-expanding period.Correlation analysis showed that expression of FPS gene was significantly positively correlated with content of Rb3(P＜0.05),and positively correlated with content of panaxtrolsaponins,but the correlation was not significant.关键词
人参/法尼基焦磷酸合成酶/生物信息技术/人参皂苷/相对表达量/相关性Key words
Panax ginseng/farnesyl diphosphate synthase/biological information technology/ginsenoside/relative quantitative expression/correlation分类
生物科学引用本文复制引用
杨林林,杨利民,马秀杰,韩梅..人参法尼基焦磷酸合成酶基因的表达及其与皂苷含量的关系[J].吉林农业大学学报,2017,39(6):695-702,708,9.基金项目
国家中药材产业技术体系岗位科学家专项(CARS-21),国家自然科学基金项目(31270371) （CARS-21）
