吉林农业大学学报2017，Vol.39Issue(6)：727-732,6.DOI:10.13327/j.jjlau.2017.3807
鹿茸再生关键组织角柄骨膜基因组DNA甲基化分析
Analysis of Genome-wide DNA Methylation in the Key Tissue (Pedicle Periosteum) for Antler Regeneration of Sika Deer
摘要
Abstract
Deer antlers are the only mammalian appendages that can fully regenerate each year from the permanent bony protuberances of the frontal bones,called pedicles.Pedicle periosteum (PP) is the key tissue for antler regeneration and the source of antler stem cells.The distal 1/3 of the PP has acquired the ability to regenerate antlers and is termed the potentiated PP (PPP),whereas the proximal 2/3 of the PP requires further interactions within its niche to launch antler regeneration,and is termed the dormant PP (DPP).However,the molecular mechanisms underlying the process of antler regeneration are unknown.In this study,we used the fluorescence-labeled methylation-sensitive amplified polymorphism method to assess the levels of DNA methylation in both cells and tissues of the PPP and the DPP.The results showed that the levels of DNA methylation were significantly lower in the PPP compared to the DPP (P＜0.05).Therefore,DNA methylation may be involved in the process of antler regeneration.This involvement was further confirmed by functional testing by artificially creating a potentiated PP (aPPP) from DPP tissue.Our data suggest that DNA methylation plays an important role in antler regeneration,and DNA demethylation is a prerequisite step for the initiation of antler regeneration,and provides the first experimental evidence to link epigenetic regulation and mammalian appendage regeneration.关键词
鹿茸/再生/致敏区角柄骨膜/休眠区角柄骨膜/DNA甲基化/F-MSAPKey words
antler/regeneration/PPP/DPP/DNA methylation/F-MSAP分类
农业科技引用本文复制引用
杨春,赵志峰,杨万云,李春义..鹿茸再生关键组织角柄骨膜基因组DNA甲基化分析[J].吉林农业大学学报,2017,39(6):727-732,6.基金项目
国家自然科学基金青年基金项目(31402059) （31402059）
