摘要
Abstract
Objective:To investigate the effects of miR-3691-3p-regulated target genes E2F3 and PRDM1 on the proliferation , migration and invasion of prostate cancer DU 145 cell line.Methods:miR-3691-3p mimic with various concentrations was transfected into DU 145 cell line, RT-PCR and Western blotting were used to detect the expression level of E 2 F3 , PRDM1 mRNA and protein .The small interfer-ing RNA scrambled siRNA, E2F3 siRNA and PRDM1 siRNA were transfected into DU145 cells.The ex-pression levels of E2F3, PRDM1 mRNA were detected by RT-PCR and MTT, Transwell and scratch tests were used to detect the proliferation , migration and invasion of DU145 cell line.Results:The expression of E2F3(P<0.01) and PRDM1(P<0.05) mRNA decreased with the increase of miR-3691-3p mimic transfection concentration in DU145 cell line, the expression level of both two protein were decreased (P<0.01).Compared with the negative control group , the expression of E2F3 and PRDM1 in DU145 cell lines transfected with E2F3 siRNA and PRDM1 siRNA were significantly decreased ( E2F3: t=31, P<0.01;PRDM1:t=10.44, P<0.01).The proliferation, migration and invasion of DU145 cells trans-fected with E2F3 siRNA, were significantly decreased ( all P <0.01 ) .The proliferation , invasion of DU145 cells transfected with PRDM 1 siRNA were significantly decreased ( all P<0 .01 ) , but the migra-tion ability was not changed(P>0.05).Conclusion:The expression of miR-3691-3p is low in DU145 cells, promoting the biological characteristics of cells by targeting E 2F3 and PRDM1.关键词
前列腺癌/DU145细胞株/miR-3691-3p/E2F3/PRDM1/生物学特性Key words
prostate cancer/DU145 cell line/miR-3691-3p/E2F3/PRDM1/biological characteristics分类
医药卫生
