兰州大学学报（医学版）2017，Vol.43Issue(6)：28-34,7.DOI:10.13885/j.issn.1000-2812.2017.06.007
6株铜绿假单胞菌噬菌体的分离及其生物学特性
Identification and biological characteristics of six Pseudomonas aeruginosa phage strains
摘要
Abstract
Objective To identify the Pseudomonas aeruginosa phage (PaP) and analyze its basic biological characteristics,such as morphology,lysis character.To determinate the multiplicity of infection (MOI),and to carry out pharmacokinetic studies of PaP 106.Methods PaP was isolated from Lanzhou city sewage samples by using 0.22 tm filtration and purified by using a double-layer agar plate.Lytic capacity of six strains of phage was measured by clinical multi drug resistant Pseudomonas aeruginosa (MDRPa).The morphology of phage106 was determined by transmission electron microscope (TEM).The host bacteria were infected with PaP106 at different MOI (10-1,10-2,10-3,10-4,10 5,10-6,and 10-7),and the optimal MOI of PaP106 was determined by the concentration of PaP.To evaluate the difference between different injection pathways (intraperitoneal,intramuscular and subcutaneous injections),and pharmacokinetic phage studies of PaP 106 were conducted.The dose of infection was 1 × 1010 pfu/mL PaP106 100 μL.The mice were dead on 0.5,1,3,6,12,24,and 36 h after intervention.The concentration of phage was measured within blood,liver,and spleen.Results Six strains of PaP had different morphologies.The PaP specifically infected and lysed between 25％-75％ bacterium isolates,and the lysis volume of PaP106 was highest (75％).The optimal MOI of PaP106 was 1 × 10-6.The time to peak of intraperitoneal injection was earlier than intramuscular injection and subcutaneous injection in blood,liver,and spleen by injecting PaP106 in vivo.Conclusion PaP106 had a powerful lysis of Pseudomonas aeruginosa.The distribution and time to peak of PaP106 was earliest in blood,liver,and spleen by intraperitoneal injection.Our study has paved a way to the further study of PaP106,and support autophage as an antibiotic for treating patients with multi drug resistant Pseudomonas aeruginosa infection in the future.关键词
铜绿假单胞菌/噬菌体/裂解谱/最佳感染复数/代谢动力学Key words
Pseudomonas aeruginosa/bacteriophages/lysis spectrum/multiplicity of infection/pharmacokinetics分类
医药卫生引用本文复制引用
张凡,陈昊,柳鹏程,王海民,李志斌,李玉民,马兴铭..6株铜绿假单胞菌噬菌体的分离及其生物学特性[J].兰州大学学报（医学版）,2017,43(6):28-34,7.基金项目
国家国际科技合作专项(2015DFA31650) （2015DFA31650）
国家级大学生创新创业训练计划项目(201410730147,20111073001501) （201410730147,20111073001501）
兰州大学大学生创新创业行动计划项目(20131073001235 （）
201410730147) （）
