临床口腔医学杂志2018，Vol.34Issue(1)：3-6,4.DOI:10.3969/j.issn.1003-1634.2018.01.002
仿生体系中评价磷灰石结合肽的矿化调控活性
Evaluating the regulating effects of an apatite binding peptide in an in vitro biomimetic mineralization system
摘要
Abstract
Objective:To evaluate the regulating effects of an apatite binding peptide on nucleation and growth of calcium phosphate nanoparticles in an in vitro biomimetic mineralization system,in which the release rate of phosphate ion is controlled by alkaline phosphatase( AP) . Methods:The cyclic peptide( NH2-CLPLWYPSC-COOH,CLP) was synthesized u-sing a solid phase method. The release rate of phosphate ion and the changes of OD820 nm were monitored in an AP-mediated biomimetic mineralization system. The morphology of the precipitates was observed under a scanning electron microscope ( SEM) . Results:Phosphate ions were continuously released within the first 30 min. The highest rate of increase in optical ab-sorbance was observed in the bovine serum albumin( BSA) group. In the presence of CLP,the increase of the absorbance was reduced and the morphology the mineralized precursors revealed a layer structure. Conclusion:The AP-mediated biomimetic mineralization system is suitable for monitoring the regulating effects of additives in initial stage of calcium phosphate precipi-tation. BSA accelerate the reaction while CLP act as an inhibitor which interacts with mineralized precursors and changes the crystal growth patterns.关键词
生物矿化/磷灰石结合肽/成核/晶体生长Key words
Biomineralization/Patite binding peptide/Nucleation/Crystal growth分类
医药卫生引用本文复制引用
张博燃,马净植,张璐瑶,杨焰,毛靖,魏蔚..仿生体系中评价磷灰石结合肽的矿化调控活性[J].临床口腔医学杂志,2018,34(1):3-6,4.基金项目
国家自然科学基金资助项目(81401524,81641035,81700961) （81401524,81641035,81700961）
