南京农业大学学报2018，Vol.41Issue(1)：71-78,8.DOI:10.7685/jnau.201703039
通过定量蛋白组学研究OsGLO1对稻瘟病抗性的调控机制
A quantitative proteomic study of the OsGLO1-mediated resistance against the rice blast disease
摘要
Abstract
[Objectives]OsGLO1(glycolate-oxidase 1)is a peroxisome-localized enzyme catalyzing hydrogen peroxide(H2O2) production. However,it remains uninvestigated whether OsGLO1 contributes to the rice defense response. Therefore,it is of great significance studying the involvement of OsGLO1 in rice defense against the blast disease,the most severe rice disease worldwide. [Methods]We compared and analyzed the rice protein expression profiles between the rice leaves inoculated by a Magnaporthe oryzae compatible(Guy11)and an incompatible strain(2539)with the mock-treated rice leaves through a quantitative proteomic approach. [Results]We found that OsGLO1 was specifically suppressed by the compatible rice blast strain but not by the incompatible strain. Guy11 is a virulent strain that causes noticeable disease symptoms on rice,whereas strain 2539 is an avirulent strain that is moderately pathogenic to many rice genotypes.Therefore,the suppressed OsGLO1 expression by Guy11 and the declined disease resistance after Guy11 infection associate OsGLO1 with blast disease resistance. By employing a rice protoplast-M. oryzae elicitor interaction system,we confirmed that the expression of OsGLO1 was positively related to rice early defense responses such as reactive oxygen species(ROS)accumulation and callose deposition. Moreover,cognate expression profiles were also observed between OsGLO1 and multiple defense signaling pathway components such as OsPBZ1,OsPAD4 and OsAOS2. [Conclusions]Our results suggest that OsGLO1 is a defense component involved in disease response against rice blast,in which multiple phytohormone signaling pathway are employed. Our study is the first case study that OsGLO1 is a critical component against rice blast disease. Considering its involvement in abiotic stress tolerance,our discovery is of great significance revealing that OsGLO1 might play mutual roles against both abiotic and biotic stresses.关键词
定量蛋白组学/稻瘟病/抗病性/葡糖酸氧化酶/过氧化氢/植物激素Key words
quantitative proteomics/rice blast/disease resistance/glycolate-oxidase/hydrogen peroxide/phytohormone分类
农业科技引用本文复制引用
于东立,宋晓欧,鲍亚林,林思远,王建升,王秀娟,卢唯,赵弘巍..通过定量蛋白组学研究OsGLO1对稻瘟病抗性的调控机制[J].南京农业大学学报,2018,41(1):71-78,8.基金项目
教育部高等学校博士点基金项目(B0201300664) （B0201300664）
江苏省自然科学基金项目(BK20141360) （BK20141360）
中央高校基本科研业务费重点项目(KYTZ201403) （KYTZ201403）
