中国海洋大学学报（自然科学版）2018，Vol.48Issue(1)：57-62,6.DOI:10.16441/j.cnki.hdxb.20160378
傅里叶变换红外光谱技术对4种志贺氏菌快速分型的研究
Rapid Typing Method for Four Species of Shigella by FT-IR Spectroscopy
摘要
Abstract
The fingerprint absorption spectra from 4 000～600 cm-1 of four species of Shigellα including S.dysenteriae,S.flexneri,S.boydii and S.sonnei were collected by FT-IR spcetroscopy,treated by baseline correction,vector normalization,and so on.The spectra data from 1 800～900 cm-1 were analyzed by two unsupervised methods of principal component analysis (PCA) and hierarchical cluster analysis (HCA) respectively to establish a rapid typing method for four species of Shigella.The spectra data library and the typing method established here were tested by HCA of target S.sonnei,S.enteritidis and E.coli seperated from contaminated pork.It was shown that a standard FT-IR spectral data library including four species of Shigella was established and could be used for the rapid typing of Shigella.Furthermore,the two methods of PCA and HCA could give good seperation of the four bacteria.The PCA and HCA results proved that the region over the range of 1 800～900 cm-1 exhibited the most representative differences,which contains three "fingerprint regions" having unique absorption,called the amide region,the mixed region and the polysaccharide region,respectively.It was also revealed that the clustering pattern obtained from HCA was in agreement with the classification obtained using PCA,indicating that the FT-IR-based methods can deliver consistent classification in spite of the multivatiate statistical analysis used.The spectra data of target S.sonnei from contaminated pork was clusterd successfully in the S.sonnei cluster of the spectra data library,but those of S.enteritidis and E.coli was clustered alonely.These results demonstrate that FT-IR spectroscopy technology combined multivariate statistical analysis is a good typing method for four species of Shigella.This study may provide a rapid,accurate and easy method for typing of Shigella,especially suitable for the basic laboratories of CDC and port quarantine departments to perform suiveillance and epidemiological traceability of Shigella.关键词
傅立叶变换红外光谱/志贺氏菌/分型/主成分分析/分级聚类分析Key words
Fourier transform infrared spectroscopy/Shigella/typing/PCA/HCA分类
生物科学引用本文复制引用
李兆杰,张玉春,刘玉敏,江艳华,王秀杰,顾华珍,李正义,杨丽君..傅里叶变换红外光谱技术对4种志贺氏菌快速分型的研究[J].中国海洋大学学报（自然科学版）,2018,48(1):57-62,6.基金项目
国家质量监督检验检疫总局科研项目(2015IK204 （）
2015IK203)资助 Supported by Science and Technology Projects of General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China (2015IK204 （）
2015IK203) （）
