山东中医杂志2018，Vol.37Issue(1)：67-70,4.DOI:10.16295/j.cnki.0257-358x.2018.01.020
高效液相色谱法同时测定还脑益聪方中α-细辛醚、β-细辛醚和细辛醛的含量
Determination Content of α-asarone,β-asarone and asarone in Huannao Yicong Formula by HPLC
摘要
Abstract
Objective:To determine the content of α-asarone,β-asarone and asarone in Huannao Yicong for-mula by HPLC. Methods:The chromatographic separations were performed on an Agilent Eclipse XDB-C18 column(250 mm×4.6 mm i.d.,5μm) with 30℃. The mobile phase consisted of methanol and 0.1％ formic acid water. The wavelength of detector was set at 265 nm,the flow rate was 1.0 mL/min. The content ofα-asarone,β-asarone and asarone was detected respectively. Results:The content of α-asarone in Rhizoma A-cori Tatarinowii and HNYCF was 1.2742 and 3.4891 mg/g,the content of β-asarone was 7.6301 and 35.836 mg/g,the content of asarone was 0.8337 and 7.8083 mg/g. Conclusion:The contents of α-asarone,β-asarone and asarone in HNYCF were 2.7,4.7 and 9.7 times than in Rhizoma Acori Tatarinowii,which indicated that the dissolution rate of the three could be increased after compatibility.关键词
高效液相色谱法/还脑益聪方/α-细辛醚/β-细辛醚/细辛醛/含量测定Key words
HPLC/Huannao Yicong formula/α-asarone/β-asarone/asarone/determination of content分类
医药卫生引用本文复制引用
梁晓东,张茜,韦云,刘龙涛,李浩..高效液相色谱法同时测定还脑益聪方中α-细辛醚、β-细辛醚和细辛醛的含量[J].山东中医杂志,2018,37(1):67-70,4.基金项目
中国博士后科学基金面上资助项目(编号:2015M571248) （编号:2015M571248）
北京市自然基金面上项目(编号:7162169) （编号:7162169）
山东省自然科学基金青年基金项目(编号:ZR2016HQ34) （编号:ZR2016HQ34）
