上海农业学报2017，Vol.33Issue(6)：1-5,5.DOI:10.15955/j.issn1000-3924.2017.06.01
来源于盐惰菌属Halopiger xanaduensis的木聚糖酶基因在毕赤酵母中诱导表达及其酶学性质的研究
Induced expression of xylanase gene from Halopiger xanaduensis in Pichia pastoris and its enzymatic properties
摘要
Abstract
The xylanase gene was designed and synthesized according to the codon preference of Pichia pastoris and xylanase protein sequence,which was connected with expression vector pYPX88 and constructed into secretory expression vector.The secretory expression vector was transformed into Pichia pastoris GS115 by electroporation after Bgl Ⅱ digestion,and the positive clones were screened out.The optimum pH,pH stability,optimum temperature and temperature stability of the recombinant xylanase,and the effects of some metal ions,sodium dodecyl sulfate (SDS),dithiothreitol (DTT)on the properties of the recombinant xylanase were studied.The results showed that the optimum pH of the recombinant xylanase was 6,and the enzyme properties were stable at pH 4.5-8.0;the optimum temperature was 60 ℃,and the enzyme properties were stable at 20-65 ℃;the metal ion Mn2+ and Cu2+ could inhibite the enzyme activity,and DTT coud promote the enzyme activity obviously.关键词
木聚糖酶/毕赤酵母/酶学性质Key words
Xylanase/Pichia pastoris/Enzymatic properties分类
生物科学引用本文复制引用
于成野,韩红娟,付晓燕,游双红,孙淼,朱恒文,彭日荷,姚泉洪..来源于盐惰菌属Halopiger xanaduensis的木聚糖酶基因在毕赤酵母中诱导表达及其酶学性质的研究[J].上海农业学报,2017,33(6):1-5,5.基金项目
上海市农业委员会重点项目(No.2011-1-8 （）
2013D-8) （）
