上海针灸杂志2018，Vol.37Issue(1)：86-91,6.DOI:10.13460/j.issn.1005-0957.2018.01.0086
电针对帕金森病模型大鼠黑质内Bip、CHOP蛋白表达的影响
Effect of Electroacupuncture on BiP and CHOP Expressions in the Substantia Nigra in a Rat Model of Parkinson Disease
摘要
Abstract
Objective To investigate the effect of electroacupuncture on the expressions of endoplasmic reticulum stress-related proteins in the substantia nigra in a rat model of rotenone-induced Parkinson disease.Methods One hundred and twenty healthy male SD rats were randomized to normal, sham operation, model, electroacupuncture pretreatment, and 7-day, 14-day, 21-day and 28-day electroacupuncture treatment groups, 15 rats each. A model of Parkinson disease was made by subcutaneous injection of rotenone in the nape. The sham operation group received an injection of equal volume of sunflower oil emulsion. The normal, sham operation and model groups were not treated. The electroacupuncture treatment group received electroacupuncture at points Fengfu (GV 16)and Taichong (LR 3) after model making. The electroacupuncture pretreatment group received model making after 7 days of electroacupuncture treatment. TH and α-syn positive cells expressions in rat substantia nigra were determined by immunohistochemical method. BiP and CHOP expressions in rat substantia nigra were examined by Western blotting. Results The model group rats had obvious syndrome characteristic of PD and rat behavior score decreased significantly after electroacupuncture intervention (P<0.01).α-syn positive cells expression and BiP and CHOP expressions in rat substantia nigra increased significantly and TH positive cells expression in rat substantia nigra decreased significantly in the model group compare with the normal and sham operation groups (P<0.01).α-syn positive cells expression and BiP and CHOP expressions in rat substantia nigra decreased significantly and TH positive cells expression in rat substantia nigra increased significantly in every electroacupuncture treatment group compare with the model group (P<0.01).Conclusions The mechanism of electroacupuncture prevention and treatment of Parkinson disease may be related to its significant inhibition of the expressions of endoplasmic reticulum stress-related proteins and protection of dopaminergic neurons.关键词
针刺疗法/帕金森病/电针/内质网应激/免疫球蛋白重链结合蛋白/CCAAT/增强子结合蛋白同源蛋白/大鼠Key words
Acupuncture therapy/Parkinson disease/Electroacupuncture/Endoplasmic reticulum stress/Immunoglobulin heavy chain binding protein/CCAAT/enhancer-binding protein homologous protein/Rats分类
医药卫生引用本文复制引用
马骏,王中明,王述菊,余沛豪,王彬,王琪..电针对帕金森病模型大鼠黑质内Bip、CHOP蛋白表达的影响[J].上海针灸杂志,2018,37(1):86-91,6.基金项目
国家自然科学基金项目(81473788,81403456) （81473788,81403456）
湖北省武汉市科学技术局高新技术产业科技创新团队计划项目(2014072704021262) （2014072704021262）
