生命科学研究2017，Vol.21Issue(6)：477-481,5.DOI:10.16605/j.cnki.1007-7847.2017.06.002
少棘蜈蚣候选杀虫肽κ-SLPTX-Ssm2e的原核表达与纯化
Prokaryotic Expression and Purification of κ-SLPTX-Ssm2e, a Candidate Insecticide from Centipede Scolopendra subspinipes mutilans
摘要
Abstract
The toxin κ-SLPTX-Ssm2e,from centipede Scolopendra subspinipes mutilans,is highly homolo-gous with a known insecticidal toxin κ-SLPTX-Ssm2a.It is composed of 31 amino acid residues and three pairs of disulfide bonds.In order to explore its structure and functions,the method of prokaryotic expression was adopted to obtain abundant toxins.The recombinant protein GST-SUMO-κ-SLPTX-Ssm2e was expressed in E.coli BL21 (DE3) by auto-induction,purified by GST affinity chromatography and digested by the Ulp1 protease to release target toxin,which was further purified by reverse-phase high performance liquid chromatography (RP-HPLC).Through the MALDI-TOF/TOF mass spectrum assay,the relative molecular mass of the polypeptide molecule was demonstrated to be 3 475.732,identical to the theoretical value 3 475.07.The research lays the foundation for further study of the insecticidal activity of κ-SLPTX-Ssm2e.关键词
κ-SLPTX-Ssm2e/杀虫肽/少棘蜈蚣/原核表达/大肠杆菌BL21(DE3)Key words
κ-SLPTX-Ssm2e/peptide insecticide/Scolopendra subspinipes mutilans/prokaryotic expression/E.coli BL21 (DE3)分类
生物科学引用本文复制引用
姚梦,赵丹,刘长军,王干,吴磊..少棘蜈蚣候选杀虫肽κ-SLPTX-Ssm2e的原核表达与纯化[J].生命科学研究,2017,21(6):477-481,5.基金项目
国防科学技术大学校预研项目(0100020414004,JC13-02-16) （0100020414004,JC13-02-16）
