生命科学研究2017，Vol.21Issue(6)：494-500,7.DOI:10.16605/j.cnki.1007-7847.2017.06.005
结核分枝杆菌Rv1057基因对巨噬细胞感染早期细胞因子表达的影响分析
Effect of Mycobacterium tuberculosis Rv1057 Gene on the Cytokine Expression in the Early Stage of Macrophage Infection
摘要
Abstract
Rv1057 is the only propeller protein in Mycobacterium tuberculosis,but its biological functions are still unclear.Here,the effects of Rv1057 gene on immune response of macrophages in the early infection with M.tuberculosis were studied.The macrophages were infected by Rv1057 deletion strain D1057 and the wild type strain H37Rv,respectively,then the multiplication rate of M.tuberculosis in macrophages was detected.Finally,the cytokine expression levcls in the early stage of macrophage infection were analyzed.The live M.tuberculosis and its multiplication rate in macrophages infected with D1057 were significantly lower than those with H37Rv.The expression levels of IL-1β,IL-10,TNF-α and IFN-γ were obviously decreased in macrophage infected by D1057.However,the expression level of IL-8 was increased,but not obviously.The experiments proved that Rv1057 deletion could reduce the immunogenicity of M.tuberculosis,and that Rv1057 was involved in the immune response between macrophages and M.tuberculosis.The experiments provide a foundation for further study in biological functions of Rv1057 and pathogenic mechanisms of M.tuberculosis.关键词
结核分枝杆菌/Rv1057基因/巨噬细胞/存活率/细胞因子/感染Key words
Mycobacterium tuberculosis/Rv 1057/macrophage/survival rate/cytokines/infection分类
生物科学引用本文复制引用
付加芳,张佩佩,古苑欣,宗工理,曹广祥..结核分枝杆菌Rv1057基因对巨噬细胞感染早期细胞因子表达的影响分析[J].生命科学研究,2017,21(6):494-500,7.基金项目
国家自然科学基金面上项目(81471921) （81471921）
山东省医学科学院医药卫生科技创新工程(201604) （201604）
