生命科学研究2017，Vol.21Issue(6)：519-522,4.DOI:10.16605/j.cnki.1007-7847.2017.06.009
高糖对海马神经元形态破坏和APP蛋白含量的影响
Effects of High Glucose on the Cellular Structures of Hippocampal Neurons and Expression of APP
摘要
Abstract
Amyloid β-protein precursor (APP) is a major molecule inducing neurodegeneration in diabetic patients.To explore the effects of high-glucose cultivation on the injury of primary hippocampal neurons and the expression of APP,the primary hippocampal neurons were separated from the embryonic day 16 (E16) SD rats and then cultured in the presence of 25 mmol/L,50 mmol/L,75 mmol/L and 100 mmol/L glucose,respectively.The cell morphology was observed with an inverted microscope and the expression of APP protein was detected by Western-blot.The results indicated that,following exposure to high concentrations of glucose,the axons of hippocampal neurons became swelling and the synapses gradually grew shorter,while the expression of APP increased.The results suggested that APP might play important roles in high glucoseinduced hippocampal neuron damage and that glycemic control would be potentially beneficial for the protection of neuronal cells.关键词
β-淀粉样蛋白前体(APP)/高血糖/神经退行性疾病/大鼠海马神经元Key words
amyloid β-protein precursor (APP)/high glucose/neurodegenerative disease/hippocampal neurons of rats分类
医药卫生引用本文复制引用
邹译娴,汤参娥,赵师,刘芬..高糖对海马神经元形态破坏和APP蛋白含量的影响[J].生命科学研究,2017,21(6):519-522,4.基金项目
国家自然科学基金资助项目(81570776) （81570776）
