生命科学研究2017，Vol.21Issue(6)：523-526,4.DOI:10.16605/j.cnki.1007-7847.2017.06.010
分泌性卷曲相关蛋白5表达上调对小鼠肝脏糖代谢的影响
Effects of Over-expression of Secreted Frizzled-related Protein 5 (SFRP5) on Glucose Metabolism in Mouse Liver
摘要
Abstract
Secreted frizzled-related protein 5 (SFRP5) is one of anti-inflammatory lipid mediators,and is found to play regulatory roles in obesity-induced diabetes.Herein,four groups of eight-week-old C57BL/6J mice were randomly assigned as normal fat diet (SD)-empty adenovirus (Ad-GFP) (n=10),normal fat diet (SD)-adenovirus over-expression SFRP5 (Ad-SFRP5) (n=10),high fat diet (HFD)-empty adenovirus (Ad-GFP) (n=10),and high fat diet (HFD)-adenovirus over-expression SFRP5 (Ad-SFRP5) (n=10).After 12 weeks,phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) mRNA and protein expression in the mice livers were measured by real-time PCR and Western-blot,respectively.The phosphorylation of insulin signaling pathway was measured by Western-blot.The results showed that both mRNA and protein expression levels of PEPCK and G6Pase were significantly decreased (P＜0.05),and the levels of phosphorylation of insulin receptors (InsR) and protein kinase B (AKT) were significantly increased in HFD-Ad-SFRP5 group (P＜0.05).Thus,over-expression of SFRP5 in C57BL/6J mice may improve insulin resistance in the liver by decreasing hepatic gluconeogenesis and enhancing the insulin signaling pathway.关键词
分泌性卷曲相关蛋白5 (SFRP5)/胰岛素抵抗/糖代谢/C57BL/6J小鼠/糖尿病Key words
secreted frizzled-related protein 5 (SFRP5)/insulin resistance/glucose metabolism/C57BL/6J mice/diabetes分类
医药卫生引用本文复制引用
朱伟,郑世霞,柯琳秋,汪毅,陈琼科,夏道涵,王红漫..分泌性卷曲相关蛋白5表达上调对小鼠肝脏糖代谢的影响[J].生命科学研究,2017,21(6):523-526,4.基金项目
重庆市基础与前沿研究计划一般项目(cstc2014jcyjA10112) （cstc2014jcyjA10112）
