水生生物学报2018，Vol.42Issue(1)：1-10,10.DOI:10.7541/2018.001
嗜水气单胞菌cpxRA缺失株构建及生物学特性研究
CONSTRUCTION AND BIOLOGICAL CHARACTERISTICS ANALYSIS OF CPXRA MUTANT IN AEROMONAS HYDROPHILA
摘要
Abstract
To investigate the roles of two Cpx component systems in the growth and virulence of Aeromonas hydro-phila, we constructed the cpxRA gene cluster mutant Δcpx with the deletion of 57—1879 bp using suicide plasmid pRE112 as the vector. This procedure was based on fusion PCR and gene homologous recombination principles. Through electrophoresis and fluorescence quantitative PCR, the partial deletion of the cpxRA gene cluster in the mutant was confirmed. Then we compared the differences between mutant and wild strains in biological characteristics includ-ing growth, biofilm formation, stress tolerance and virulence. The results showed that the mutant had no signifi-cant difference with the wild strain in morphology, growth, biofilm formation and virulence. The main differences exis-ted in the response to high osmotic pressure, SDS (Sodium dodecyl sulfate), EDTA (Ethylene Diamine Tetraacetic Acid) and polymyxin B stimulation. This study reveals that the Cpx system of Aeromonas hydrophila is involved in the response to external stimulus factors, and plays a relatively minor role in virulence.关键词
嗜水气单胞菌/cpxRA/pRE112/应激耐受Key words
Aeromonas hydrophila/cpxRA/pRE112/Stress tolerance分类
农业科技引用本文复制引用
张兰梨,张倩倩,陈辉,王谢昊,吴振兵,冯宇晴,李爱华..嗜水气单胞菌cpxRA缺失株构建及生物学特性研究[J].水生生物学报,2018,42(1):1-10,10.基金项目
通威股份有限公司国际合作计划项目(2015HH0070) （2015HH0070）
江苏省海洋与渔业局项目(D2015-11) （D2015-11）
国家重点实验室自主创新课题(2016FBZ04)资助 [Supported by Tongwei Group Co., Ltd project (2015HH0070) （2016FBZ04）
Jiangsu Marine and Fisheries Bureau Project (D2015-11) （D2015-11）
FEBL Project (2016FBZ04)] （2016FBZ04）
