水生生物学报2018，Vol.42Issue(1)：11-16,6.DOI:10.7541/2018.002
草鱼肠道黏膜厌氧细菌的分离与鉴定
ISOLATION AND IDENTIFICATION OF ANAEROBES IN THE INTESTINAL MUCOSA OF GRASS CARP CTENOPHARYNGODON IDELLUS
摘要
Abstract
The bacteria community in the gastrointestinal tract of fish has been the subject of numerous studies. However, most of those researches have focused on the molecular ecology of intestinal microbiota. Few studies pay at-tention to the bacterial culture, especially to the culture of the anaerobic intestinal bacteria, which leads to poor descrip-tion in anaerobic autochthonous bacteria of gut. In this study, we investigated the autochthonous microflora of intesti-nal mucosa in grass carp (Ctenopharyngodon idellus) with the culture dependent method. The bacterial numbers in foregut, midgut and hindgut of the fish were 3.17×104, 1.63×105 and 1.79×108 cfu/g, respectively. A total of 274 bac-terial isolations were sequenced and identified as genus Bacteroides, Cetobacterium, Fusobacterium, Aeromonas, Shewane-lla, Bacilluss, Pantoea and Citrobacter. 9.1％ of which were obligate anaerobic bacteria and the others were faculta-tive bacteria. The anaerobic bacteria, Bacteroides paurosaccharolyticus and Bacteroides. Eggerthii, were only found in midgut and hindgut, while Cetobacterium somerae and Fusobacterium varium were only found in hindgut. The facul-tative bacteria existed in the whole gut, among which Aeromonas hydrophila was dominant. Our results suggest that species and quantities of intestinal bacterial vary with the segments of C. idellus, and hindgut presents higher bacterial abundance and diversity.关键词
草鱼/肠道黏膜/厌氧细菌/细菌丰度Key words
Ctenopharyngodon idellus/Intestinal mucosa/Anaerobic bacteria/Bacteria abundance分类
生物科学引用本文复制引用
丰文雯,吴山功,郝耀彤,李文祥,李明,邹红,王桂堂..草鱼肠道黏膜厌氧细菌的分离与鉴定[J].水生生物学报,2018,42(1):11-16,6.基金项目
国家自然科学基金 (31372571和31272706)资助 [Supported by the National Natural Science Foundation of China (31272706, 31372571)] （31372571和31272706）
