水生生物学报2018，Vol.42Issue(1)：17-25,9.DOI:10.7541/2018.003
中华绒螯蟹肝胰腺坏死综合症病原及病理学研究
ETIOLOGICAL AND HISTOPATHOLOGICAL STUDY ON HEPATOPANCREATIC NECROSIS SYNDROME IN ERIOCHEIR SINENSIS
摘要
Abstract
A disease called "Shuibiezi" in Eriocheir sinensis was prevailed on Xinghua city, Jiangsu province since 2015, and slowly spread to other main areas of crab rearing in the whole nation. To explore the causes, the etiology by parasites examination, pathogenic bacteria isolation, challenge experiment and electron microscopy observation, and the histopathology by routine paraffin-section technique were conducted with healthy E. sinensis as control groups. Results showed that no pathogenic microorganism was detected by etiology research. Pathological changes with diffe-rent degrees were observed in gills, muscles and hepatopancreas of diseased crabs, and the main pathological characteri-stics were gill tissue thickening, enlarged gill cavity, increased number of hemocyte, and marginalized nucleus. Vacuo-lization of the epithelial cells of the hepatopancreas and transferred vacuoles appeared. With the severity of the condi-tions, hepatic cells were irregular-arranged, and the number and volume of vacuoles and transferred vacuoles increased, and the number of granular materials in transferred vacuoles increased. With the most severe disease, the hepatic tubles were so ruptured that substances in cells were leaked, and the nucleus of hepatic cells were broken and hepatic cells ap-peared necrosis. The main lesion characteristics of muscle were muscular fibers appeared to be relaxed and pyknotic of nuclei. Etiology and incidence investigation revealed that the disease was caused by non living agents. Based on the main pathological characteristics and pathogenesis, this disease nominate scientifically as hepatopancreatic necrosis syndrome.关键词
中华绒螯蟹/肝胰腺坏死综合症/病原学/病理学Key words
Eriocheir sinensis/Hepatopancreatic necrosis syndrome/Etiology/Histopathology分类
农业科技引用本文复制引用
杨宗英,张一柳,胡鲲,刘力硕,蔡红桂,张凤翔,杨先乐..中华绒螯蟹肝胰腺坏死综合症病原及病理学研究[J].水生生物学报,2018,42(1):17-25,9.基金项目
河蟹"水瘪子"病控制技术研究(A1-2037-17-000114)资助 [Supported by the research on control technology of "shuibiezi" diseased Eriocheir sinensis] （A1-2037-17-000114）
