水生生物学报2018，Vol.42Issue(1)：26-32,7.DOI:10.7541/2018.004
棘胸蛙致病性蜡样芽孢杆菌的分离鉴定及病理组织观察
ISOLATION AND IDENTIFICATION OF BACILLUS CEREUS AND PATHOLOGICAL OBSERVATION FROM ROTTEN-SKIN DISEASE OF QUASIPAA SPINOSA
摘要
Abstract
Rotten-skin disease is very common in the artificial culture of the giant spiny frog (Quasipaa spinosa). To screen pathogenic bacteria that cause illness of giant spiny frog, gram stain, morphological observation, physiological, and biochemical identification, and sequence analysis of 16S rDNA were performed and three bacteria (BS1, BS2, and BS3) were identified. The results showed that BS1, BS2, and BS3 are Bacillus cereus. Liver, lung, tongue, and muscle were impaired with degeneration, necrosis, and inflammatory cell infiltration. Moreover, basophilic inclusions were ob-served in the lung of diseased giant spiny frog. The drug sensitivity test showed that all three pathogenic bacteria were highly sensitive to gentamicin and streptomycin, moderate sensitive to tobramycin, kanamycin and tetracycline, and resistant to ampicillin and ceftazidime. This study explored pathogenesis of rotten-skin disease of giant spiny frog, would provide theoretical basis for prevention and cure of rotten-skin disease of giant spiny frog.关键词
棘胸蛙/烂皮病/蜡样芽孢杆菌/组织病理/药敏试验Key words
Quasipaa spinosa/Rotten-skin disease/Bacillus cereus/Histopathology/Drug sensitivity test分类
农业科技引用本文复制引用
吕耀平,金晶,施倩,陈洁,刘子明,陆君,黄富友,傅旭豪..棘胸蛙致病性蜡样芽孢杆菌的分离鉴定及病理组织观察[J].水生生物学报,2018,42(1):26-32,7.基金项目
国家自然科学基金(31500308) （31500308）
浙江省"十三五"一流学科生态学(B类) （B类）
浙江省自然基金(Y3110477) （Y3110477）
浙江省新苗人才计划(2015R429016) （2015R429016）
浙江省丽水市科技局项目(20150407)资助 [Supported by the National Natural Science Foundation of China (31500308) （20150407）
the First-class Disciplines of Ecology of Zhejiang Province of China （）
the Natural Science Foundation Zhejiang Province of China (Y3110477) （Y3110477）
the Young Talent Fostering Program of Zhejiang Province (2015R429016) （2015R429016）
the Key Project of Lishui Science &Technology of Zhejiang Province of China (20150407)] （20150407）
