水生生物学报2018，Vol.42Issue(1)：33-38,6.DOI:10.7541/2018.005
青海湖裸鲤舌状绦虫裂头蚴的分子鉴定及系统发育研究
MOLECULAR IDENTIFICATION AND PHYLOGENETIC STUDY OF LIGULA INTESTINALIS PLEROCEROID IN GYMNOCYPRIS PRZEWALSKII FROM THE QINGHAI LAKE, CHINA
摘要
Abstract
To identify Ligula intestinalis pleroceroid parasitizing in the abdominal cavity of Gymnocypris przewalskii and analyze its phylogenic status from Qinghai Lake, China, polymerase chain reaction (PCR) of 18S ribosomal RNA gene, COⅠand COB gene was carried out. Their sequences homologies with other tapeworm species were analyzed and the phylogenetic relationships were inferred. The results showed that the noodle-like parasite was identified as L. intestinalis, and that its 18S rRNA gene homogeneity was 100.00％ with AF254121 (China), and COⅠand COB gene homogeneities were 99.49％ and 93.32％ with AF153910 (China) and JQ279109 (Algeria), respectively. Furthermore, the phylogenetic trees based on the 18S rRNA, COⅠand COB gene sequences indicated that the collected tapeworm was in the same branch with L. intestinalis, which are close to the isolated species from China. The phylogenetic rela-tionship between the identified L. intestinalis and other tapeworm species was elucidated.关键词
青海湖/裸鲤/舌状绦虫/分子鉴定/系统发育Key words
Qinghai Lake/Gymnocypris przewalskii/Ligula intestinalis/Molecular identification/Phylogeny分类
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张学勇,简莹娜,李秀萍,裴全邦,王戈平,王光华,蔡其刚,马利青..青海湖裸鲤舌状绦虫裂头蚴的分子鉴定及系统发育研究[J].水生生物学报,2018,42(1):33-38,6.基金项目
绒毛用羊疾病防控(治) 关键技术研究与示范(CARS-39-6B) （治）
国家外国专家局"千人计划"高层次外国专家项目(WQ20136300172)资助 [Supported by the Project of Research and Demonstration of Key Technologies For Prevention and Treatment of Diseases of Wool Sheep and Cashmere Goat (CARS-39-6B) （WQ20136300172）
SAFEA: "1000 Talent Plan for High-Level Foreign Experts" Program (WQ20136300172)] （WQ20136300172）
