水生生物学报2018，Vol.42Issue(1)：57-67,11.DOI:10.7541/2018.008
灌喂氧化鱼油后黄颡鱼胃肠道黏膜黑色素细胞分化和黑色素合成途径基因的差异表达
TRANSCRIPTOME ANALYSIS OF MELANOCYTE DIFFERENTIATION AND THE MELANIN SYNTHESIS METABOLIC PATHWAY IN THE GASTROINTESTINAL MUCOSA OF YELLOW CATFISH (PELTEOBAGRUS FULVIDRACO) FED OXIDIZED FISH OIL
叶元土 1汪冰 1张宝彤 2萧培珍 2吴萍 1蔡春芳 1吴代武 1罗琪刚 1何杰 1高敏敏 1周露阳 1王梦影1
作者信息
- 1. 苏州大学基础医学与生物科学学院, 江苏省水产动物营养重点实验室, 苏州 215123
- 2. 北京营养源研究所水产动物系统营养研究开放实验室, 北京 100000
- 折叠
摘要
Abstract
Transcriptome analysis of yellow catfish (Pelteobagrus fulvidraco) by RNA-seq method extracting gastrointestinal mucosa Total RNA was studied in this paper. The fish were fed with oxidized fish oil and fish oil re-spectively for 7d. Differential expression of genes related to melanin synthesis, melanosomes movement, melanocyte stimulating hormone-α pathway and three signal pathways controlling melanocyte differentiation were analyzed. The results showed that there were metabolic networks of melanocyte differentiation and development, melanin synthesis and regulation in the gastrointestinal mucosa. The genetic information of key enzymes and protein of melanin synthesis were introduced with plotting metabolic networks graph. The melanin content was deficient because of the genes ex-pression level were significantly down-regulation related to controlling melanin synthesis after fed with oxidized fish oil. While melanocyte differentiation and development were improved because of the genes expression level were sig-nificantly up-regulation related to melanocyte stimulating hormone-α pathway (α-MSH). In the same time, genes ex-pression level of WNT/β-catenin, EDN3 / EDNRB and KIT/KITL signal pathways were different. In conclusion, feed-ing oxidized fish oil affected melanocyte differentiation and development and reduced melanocyte number which be-nefited to maturity in yellow catfish. Insufficient melanin biosynthesis also led to body color variation of yellow catfish.关键词
黑色素/黑素体/黑色素细胞/胃肠道黏膜/黄颡鱼Key words
Melanin/Melanosomes/Melanocytes/Gastrointestinal mucosa/Catfish分类
生物科学引用本文复制引用
叶元土,汪冰,张宝彤,萧培珍,吴萍,蔡春芳,吴代武,罗琪刚,何杰,高敏敏,周露阳,王梦影..灌喂氧化鱼油后黄颡鱼胃肠道黏膜黑色素细胞分化和黑色素合成途径基因的差异表达[J].水生生物学报,2018,42(1):57-67,11.
