水生生物学报2018，Vol.42Issue(1)：77-85,9.DOI:10.7541/2018.010
尼罗罗非鱼Ikaros基因的克隆及表达分析
MOLECULAR CLONING AND EXPRESSION ANALYSIS OF IKAROS GENE FROM NILE TILAPIA (OREOCHROMIS NILOTICUS)
摘要
Abstract
Ikaros is a kind of transcription factor with zinc finger structure that is essential to the development of lymphocyte. In order to reveal the structure characteristic of Ikaros gene and the immune modulatory mechanisms of Ikaros gene on disease resistant, the cDNA of Ikaros were obtained from Oreochromis niloticus with RT-PCR and RACE methods, and the genomic DNA of Ikaros were obtained by using PCR and Genome Walking technique in this study. Quantitative real-time PCR was used for analyzing the organization distribution and the response of Ikaros to Streptococcus agalactiae infection. The genomic DNA is 20545 bp with 7 introns and 8 exons encoding 6 kinds of mRNA splicing isoform via alternative splicing. The deduced amino acid sequences of the six splicing isoforms with zinc finger domain have high homology (70.6％—93.7％) in other teleost fish. The Ikaros gene expressed in all tested tissues, which strongly expressed in blood, and moderately expressed in thymus, spleen and head kidney. After chal-lenged with pathogenic bacteria Streptococcus agalactiae, the Ikaros gene expression levels rose in blood, thymus, spleen and head kidney, and then reached to the peak after 48 hours. The results suggested that Ikaros gene participated in the immune response of Nile tilapia against Streptococcus agalactiae infection. This research laid a foundation for further study on the disease resistant mechanism of Ikaros gene.关键词
尼罗罗非鱼/Ikaros基因/mRNA剪接异构体/无乳链球菌/表达Key words
Oreochromis niloticus/Ikaros gene/mRNA splicing isoform/Streptococcus agalactiae/Expression分类
生物科学引用本文复制引用
陈昆平,衣萌萌,刘志刚,卢迈新,高风英,张德锋,可小丽,曹建萌,朱华平,王淼..尼罗罗非鱼Ikaros基因的克隆及表达分析[J].水生生物学报,2018,42(1):77-85,9.基金项目
现代农业产业技术体系专项资金(CARS-46) （CARS-46）
广东省自然科学基金(2016A030313146) （2016A030313146）
国家自然科学基金(31502205)资助[Supported by China Agriculture Research System (CARS-46) （31502205）
the National Science Foundation of Guangdong Province (2016A030313146) （2016A030313146）
the National Natural Science Foundation of China (31502205)] （31502205）
