水生生物学报2018，Vol.42Issue(1)：86-93,8.DOI:10.7541/2018.011
脊尾白虾血蓝蛋白大亚基基因的克隆及表达分析
cDNA CLONING AND EXPRESSION ANALYSIS OF HEMOCYANIN SUBUNIT GENE IN EXOPALAEMON CARINICAUDA
摘要
Abstract
This study cloned the cDNA of hemocyanin subunit from Exopalaemon carinicauda (named EcHcL) by ra-pid amplification of cDNA ends (RACE). The EcHcL cDNA was 2192 bp in length including a 2034 bp open reading frame (ORF) that encoded a 667 amino acids polypeptide with a signal peptide sequence (1—21 amino acid residues). The predicted molecular weight of the mature peptide was 78.5 kD. The homology analysis revealed that the amino acid sequence of EcHcL was highly conserved with other arthropod. The homology similarities of EcHcL amino acids with Macrobarchium nipponense hemocyanin and Litopenaeus vannamei amino acid sequence were 87％ and 73％, re-spectively. The similarity of the M domain of the amino acid sequence between EcHcL and other shrimp was about 90％. EcHcL was detected in all tested tissues including gill, ovary, hepatopancreas, heart, intestine, muscle, stomach, abdominal ganglion, eyestalk and haemocytes with the highest expression in hepatopancreas. EcHcL was induced dis-tinctly in the hepatopancreas and haemocytes of shrimp by Staphylococcus aureus, Vibrio parahaemolyticus and white spot syndrome virus (WSSV). The results suggest that the different temporal expression pattern of EcHcL might play an important role in shrimp immune system.关键词
脊尾白虾/血蓝蛋白大亚基/基因克隆/表达分析Key words
Exopalaemon carinicauda/Hemocyanin subunit/Cloning/Expression analysis分类
农业科技引用本文复制引用
窦全伟,李吉涛,刘萍,李健,刘九美,孙东方,蔡影,环朋朋..脊尾白虾血蓝蛋白大亚基基因的克隆及表达分析[J].水生生物学报,2018,42(1):86-93,8.基金项目
国家虾产业技术体系(CARS-47) （CARS-47）
泰山产业领军人才工程(LJNY2015002) （LJNY2015002）
国家自然科学基金(31472275) （31472275）
青岛市产业培育计划(科技惠民专项)(17-3-3-62-nsh) （科技惠民专项）
宁波市科技计划 (2016C11011)资助 [Supported by the Earmarked Fund for Modern Agro-industry Technology Research System (CARS-47) （2016C11011）
the Program of Shandong Leading Talent (LNJY2015002) （LNJY2015002）
the National Natural Science Foundation of China (31472275) （31472275）
Qingdao Industrial Development Program (Science and Technology Benefit Special Project) (17-3-3-62-nsh) （Science and Technology Benefit Special Project）
Projects of Science and Technology of Ningbo (2016C11011)] （2016C11011）
