水生生物学报2018，Vol.42Issue(1)：94-98,5.DOI:10.7541/2018.012
中华鲟促性腺激素释放激素受体基因cDNA序列的克隆及表达分析
CLONING AND EXPRESSION ANALYSIS OF GONADOTROPIN-RELEASING HORMONE RECEPTOR, GNRH-R IN CHINESE STURGEON (ACIPENSER SINENSIS)
摘要
Abstract
The Chinese sturgeon (Acipenser sinensis), an endangered and national protected species, is an important re-source for sturgeon aquaculture industry. This study cloned the full-length cDNA of the gonadotropin-releasing hor-mone receptor (GnRH-R) from pituitary cDNA library of a 24 years old female Chinese sturgeon using rapid amplifica-tion of cDNA ends (RACE). The nucleotide sequences of AsGnRH-R is 1530 bp in length, containing a 478 bp 5′-un-translated region (UTR), a 473 bp 3′-UTR, and a 579 bp open reading frame (ORF) with a mature peptide of 192 amino acids encompassing 5 putative N-glycosylation sites. Multiple sequence alignment and phylogenetic analysis of the de-duced amino acid sequence of AsGnRH-R indicated that the most similar ortholog was red sea bream (Pagrus major) (76％), and that the least similar ortholog was Callorhinchus milii (39％). Real-time quantitative PCR analysis indica-ted that the AsGnRH-R was expressed in tissues including liver, spleen, kidney, testis, muscle, intestine, and brain with the highest level in the testis.关键词
中华鲟/GnRH-R/基因克隆/基因表达Key words
Chinese sturgeon/GnRH-R/Gene cloning/Gene expression分类
生物科学引用本文复制引用
王桂苹,杜合军,冷小茜,李创举,曹宏..中华鲟促性腺激素释放激素受体基因cDNA序列的克隆及表达分析[J].水生生物学报,2018,42(1):94-98,5.基金项目
三峡工程鱼类资源保护湖北省重点实验室开放课题(0704103)资助 [Supported by the Institute of Chinese Sturgeon Research, China Three Gorges Project Corporation, Grants from the Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes (0704103)] （0704103）
