水生生物学报2018，Vol.42Issue(1)：196-205,10.DOI:10.7541/2018.025
我国新记录海链藻属物种的形态学和系统学研究
THE MORPHOLOGICAL AND PHYLOGENETIC STUDIES ON TWO NEWLY RECORDED THALASSIOSIRA SPECIES FROM CHINA
摘要
Abstract
To investigate the species diversity of Thalassiosira in China and provide molecular data for further phylo-genetic studies, monoclonal strains have been isolated and established from some representative sites in Chinese coast. The morphology was studied using light and electron microscopy, and the hypervariable D1–D3 region of the nuclear large subunit ribosomal RNA-encoding gene region (LSU rRNA gene) was amplified and sequenced. Two newly recor-ded Thalassiosira species (T. anguste-lineata (Schmidt) Fryxell & Hasle and T. minicosmica Lee & Park) for China were reported based on the combination of morphology and molecular data. Their detailed morphology were described and compared with allied taxa. The results of molecular phylogenetic tree based on sequences data of D1—D3 region of LSU rRNA indicated that neither T. anguste-lineata nor T. minicosmica were clustered with T. nordenskioeldii, the type species of the genus Thalassiosira, suggesting great genetic difference is present among Thalassiosira taxa, and that the present morphology-based genus Thalassiosira is not a natural group because it is separated by some other allied genus, such as Skeletonema, Cyclotella, Planktoniella. These results revealed that the present reported Thalassiosira species should be paraphyletic group and will be gradually revised into multiple independent genera in future phylogenetic study. However, it is difficult to carry out specific taxonomic revisions because of limited molecular data that is an im-portant constraint for the advancement of related researches.关键词
狭线形海链藻/碟形海链藻/新记录/海链藻属/形态学/系统学Key words
Thalassiosira anguste-lineata/Thalassiosira minicosmica/Newly record/Thalassiosira/Morphology/Phylogenetics分类
农业科技引用本文复制引用
郭雅琼,李扬..我国新记录海链藻属物种的形态学和系统学研究[J].水生生物学报,2018,42(1):196-205,10.基金项目
国家自然科学基金(31370235和31570205) （31370235和31570205）
广东省科技计划项目(2015A030401084)资助 [Supported by National Natural Science Foundation of China (31370235, 31570205) （2015A030401084）
Science and Technology Foundation of Guangdong Province (2015A030401084)] （2015A030401084）
