生态学报2017，Vol.37Issue(24)：8361-8373,13.DOI:10.5846/stxb201611012225
氮磷添加对亚热带常绿阔叶林土壤微生物群落特征的影响
Effects of nitrogen and phosphorus addition on soil microbial community characteristics in a subtropical evergreen broadleaved forest
摘要
Abstract
To reveal the effects of nitrogen and phosphorus addition on soil microbial characteristics,an old-growth evergreen broadleaved forest in the Xianyu Mountains in Chizhou of the Anhui Province was selected.Four treatments of nitrogen and phosphorus addition were designed,including a control (CK,0 kg N/hm2),low nitrogen (LN,50 kg N/ hm2),high nitrogen (HN,100 kg N/hm2),and high nitrogen + phosphorus (HN + P,100 kg N/hm2+ 50 kg P/hm2) treatments.Microbial biomass carbon (MBC),biomass nitrogen (MBN),and microbial community functional diversity for different soil layers (0-10 cm,10-20 cm,and 20-30 cm) were analyzed using the methods of chloroform-fumigation and the Biolog system.The results showed that MBC and MBN decreased with soil depth,with significant differences among soil layers (P ＞ 0.05).The MBC and MBC/MBN ratio changed significantly after N and P addition.The metabolic activity of the soil microbial community also declined with soil depth.Soil microbial activities were highest in the HN and LN treatments.Mclntosh,Shannon,and Simpson diversity indices differed in different soil layers and at different dosages of N and P.Microbial diversity indices for the 0-10 cm soil layer were significantly different among treatments.Carbon source utilization by soil microbes was highest for carboxylic acids,amino acids,and carbohydrates.The results of principal component analysis showed that carbon source utilization was different among the soil layers.There was significant spatial variability in carbon source utilization in the upper 0-10 cm of soil under different N and P treatments,whereas it was relatively stable in other soil layers (10-30 cm).This spatial heterogeneity was mainly manifested in carbon source utilization of carbohydrates and carboxylic acid.In general,the soil layer and N and P treatments had significant impacts on MBC,MBN,and soil microbial functional diversity,with greatest impacts occurring on the surface soil with high N treatment.关键词
氮磷添加/常绿阔叶林/土壤微生物群落/功能多样性/微生物量碳、氮Key words
nitrogen and phosphorus addition/evergreen broadleaved forest/soil microbial community/functional diversity/microbial biomass C and N引用本文复制引用
王晶晶,樊伟,崔珺,许崇华,王泽夫,徐小牛..氮磷添加对亚热带常绿阔叶林土壤微生物群落特征的影响[J].生态学报,2017,37(24):8361-8373,13.基金项目
国家自然科学基金项目(31370626,31070588) （31370626,31070588）
国家“973”计划项目(2012CB416905) （2012CB416905）
