生物加工过程2018，Vol.16Issue(1)：38-48,11.DOI:10.3969/j.issn.1672-3678.2018.01.005
酰胺酶的挖掘及在有机合成中的应用进展
Advances in exploitation and application of amidase in organic synthesis
摘要
Abstract
Amidases are ubiquitous enzymes,and hydrolyze the C-N bonds of amide compounds to produce corresponding carboxylic acids with release of ammonia. Amidases play important roles in fine chemicals synthesis, pharmaceutical and agrochemical industries due to their broad substrate spectrum and remarkable chemo-,regio-,and enantio-selectivity. Amidases can be assigned into two families according to their amino acid sequences:the amidase signature ( AS) family and the nitrilase superfamily. Here,we systematically review the advances in studying amidase structures and catalytic mechanisms,and describe their applications in pharmaceutical and agrochemical synthesis.关键词
酰胺酶/酰胺酶标签家族/腈水解酶家族/生物催化Key words
amidase/nitrilase superfamily/amidase signature family/biocatalysis分类
生物科学引用本文复制引用
吴哲明,金建强,郑仁朝..酰胺酶的挖掘及在有机合成中的应用进展[J].生物加工过程,2018,16(1):38-48,11.基金项目
浙江省自然科学基金( Q18B060021) （ Q18B060021）
