生物加工过程2018，Vol.16Issue(1)：59-66,8.DOI:10.3969/j.issn.1672-3678.2018.01.007
无细胞蛋白质合成的研究进展
Advances in cell-free protein synthesis
张鹏 1林骏 2卢元1
作者信息
- 1. 清华大学 化学工程系,北京 100084
- 2. 中国石油大学(北京) 新能源研究院,北京 102249
- 折叠
摘要
Abstract
Cell-free synthetic biology as an emerging interdisciplinary approach,has been well adopted for protein engineering.Cell-free biosynthesis system activates the transcription and translation machinery in vitro without using intact living cells,so it can decrease the dependence on cells,and leads to an increase in the engineering flexibility.These advantages of cell-free systems have allowed them to become a robust protein engineering platform for the synthesis of membrane proteins and therapeutic proteins,incorporation of unnatural amino acids, and high-throughput screening. Cell-free protein synthesis system holds great promises for applications ranging from basic science to industrial biomanufacturing. In this paper, the research progress, applications and future prospect of cell-free protein synthesis systems have been systematically reviewed.关键词
无细胞合成生物学/无细胞蛋白质合成/蛋白质工程/非天然氨基酸/高通量分析Key words
cell-free synthetic biology/cell-free protein synthesis/protein engineering/unnatural amino acids/high-throughput analysis分类
生物科学引用本文复制引用
张鹏,林骏,卢元..无细胞蛋白质合成的研究进展[J].生物加工过程,2018,16(1):59-66,8.
