生殖医学杂志2018，Vol.27Issue(1)：76-81,6.DOI:10.3969/j.issn.1004-3845.2018.01.015
棕榈酸诱导人绒毛滋养层细胞胰岛素抵抗模型的建立
Establishment of palmitic acid induced insulin resistance model in human villous trophoblast cells
摘要
Abstract
Objective:To investigate whether the high palmitic acid(PA)can induce the placental trophoblast cells(HTR8-S/Vneo)to form insulin resistance in high fat environment.Methods:The optimum concentrations of palmitic acid and insulin which do not affect the proliferation of HTR8-S/Vneo cells were tested by MTT assay.According to the optimum concentrations obtained by the MTT assay,the HTR8-S/Vneo cells were divided into four groups(control group,PA group,insulin group,PA-insulin co-treatment group).The cell glucose uptake and intracellular total sugar were determined in the different treatment conditions and different processing time.Results:The MTT assay in the HTR8-S/Vneo cells treated with different concentrations of PA and insulin found that PA concentration of 0.125 mmol/L and insulin concentration of 100 nmol/L had no significant effect on cell proliferation(P＞0.05),so the concentration of PA and insulin was chosen to treat HTR8-S/Vneo cells.Glucose consumption and total intracellular total sugar were measured at 12,18,24 and 36 hours after treatment.The experiment results found glucose consumption and intracellular total sugar were increased after insulin treatment compared with the control group(P＜0.05),but there was no significant changes in glucose consumption and intracellular total sugar content after PA treatment(P＜0.05).Compared with PA treatment alone group,PA-insulin synergistic treatment group could significantly increase cells at 12 and 18 hours(P＜0.05),but the significant difference was disappeared at 24 and 36 hours(P＞0.05).Compared with insulin alone group,glucose consumption and total cellular sugar were significantly reduced in PA-insulin co-treatment group(P＜0.05).Conclusions:The synergistic effect of palmitic acid and insulin for 24 hours results in the decrease of insulin sensitivity in HTR8-S/Vneo cells to establish the insulin resistance model in HTR8-S/Vneo cells.Compared with the reported model,palmitic acid-insulin synergistic treatment can greatly shorten the time of insulin resistance in cells.关键词
人绒毛滋养层细胞/棕榈酸/胰岛素抵抗Key words
HTR8-S/Vneo/Insulin resistance/Palmitic acid引用本文复制引用
管纯一,马旭,夏红飞..棕榈酸诱导人绒毛滋养层细胞胰岛素抵抗模型的建立[J].生殖医学杂志,2018,27(1):76-81,6.基金项目
国家自然科学基金(81370720) （81370720）
中央级公益性科研院所基本科研业务费专项(2015GJZ01) （2015GJZ01）
