信阳师范学院学报（自然科学版）2018，Vol.31Issue(1)：40-45,6.DOI:10.3969/j.issn.1003-0972.2018.01.008
家蚕非编码RNA Bm-152功能初探
Preliminary Functional Analysis of Noncoding RNA Bm-152 of Bombyx Mori
摘要
Abstract
Non-coding RNA Bm-152 accumulated more in silk gland of silkworm Bombyx mori.Real-time PCR was used to study the expression levels of Bm-152 in the silk glands of silkworm from the fifth instar lar-vae to prepupa stage.Furthermore,the signaling pathway in which Bm-152 may participate were studied as Bm-152 was overexpressed in the 3 r d day of the fifth larva of silkworm.Results showed that Bm-152 had no obvious change during the development of silk glands of the fifth instar larvae of silkworm,Bm-152 had more expression at the first spinning day in the silk gland,then decreased in the pre-pupa stage.After 48h injection of 2μg dose of piggyBac [A3-EGFP-A3-Bm-152]overexpression vector,it was found that Bm-152 was not overexpressed in the silk gland,but was significantly overexpressed in the non-silk gland,and the expression of Bm-152 increased 1.66 times.Moreover,the expression of Usp (ecdysone signaling pathway)increased by 1.95 times in the non-silkgland tissues,E75A (ecdysone signaling pathway)increased by 2.26 times,and the expression of Met(ju-venile hormone signaling pathway)increased by 1.79 times in the non-silkgland tissues.Bm-152 might participate in the development of silkworm through the ecdysone and juvenile hormone signaling pathway.These results laid foundations for further study of functions of Bm-152 in silkworm.关键词
家蚕/丝腺/非编码RNA/Bm-152/表达谱Key words
Bombyx mori/silk gland/non-coding RNA/Bm-152/expression profiles分类
生物科学引用本文复制引用
刘晓,邱妩洁,李新梅,阚云超,李丹丹..家蚕非编码RNA Bm-152功能初探[J].信阳师范学院学报（自然科学版）,2018,31(1):40-45,6.基金项目
国家自然科学基金项目(31100938,31470114) （31100938,31470114）
