中国病理生理杂志2018，Vol.34Issue(1)：47-51,5.DOI:10.3969/j.issn.1000-4718.2018.01.008
bFGF通过促进心脏血管新生改善心肌梗死后小鼠心脏重构
Effect of bFGF on promoting angiogenesis in infarct area and improving myocardial fibrosis in mouse myocardial infarction model
摘要
Abstract
AIM:To investigate the protective effect of basic fibroblast growth factor(bFGF)on the heart of mice with myocardial infarction and its mechanism.METHODS:The model of myocardial infarction was established by the ligation of left anterior descending artery of C57/B6 mice(8～12 weeks old)after lateral thoracotomy.The mice were divided into sham operation group ,myocardial infarction group and bFGF administration group.bFGF at 0.5μg was intra-peritoneally injected on alternate days after myocardial infarction for 7 d.Cardiac Doppler ultrasonography was used to de-tect cardiac function after myocardial infarction for 28 d,and left ventricular end-diastolic diameter ,left ventricular end-systolic diameter,left ventricular ejection fraction and left ventricular shortening fraction(LVFS)were used to evaluate cardiac function.After myocardial infarction for 28 d,the mice were sacrificed and the hearts were collected for preparing pathological sections.The degrees of myocardial fibrosis and angiogenesis in the myocardial infarction area were observed. Western blot was used to detect the indicators of angiogenesis.RESULTS:The results of Masson staining showed that bF-GF administration significantly reduced myocardial fibrosis at 28 d after myocardial infarction.Cardiac ultrasound data showed that cardiac functions in myocardial infarction group were poorer than those in sham group ,and bFGF administration significantly improved cardiac functions.Immunofluorescence staining showed that neovascularization in myocardial infarc -tion area of bFGF administration group was more than that in myocardial infarction group.The results of Western blot showed that bFGF activated AKT/HIF-1α/VEGF signaling pathway.CONCLUSION:Intraperitoneal injection of bFGF reduces myocardial fibrosis and improves cardiac function in myocardial infarction mice.bFGF may promote angiogenesis by activating AKT/HIF-1α/VEGF signaling pathway.关键词
碱性成纤维细胞生长因子/心肌梗死/血管新生/AKT/HIF-1α/VEGF信号通路/心肌纤维化Key words
Basic fibroblast growth factor/Myocardial infarction/Angiogenesis/AKT/HIF-1α/VEGF signa-ling pathway/Myocardial fibrosis分类
医药卫生引用本文复制引用
沈丹萍,戈东辉,陈显达,吴雨晴,李磊,褚茂平..bFGF通过促进心脏血管新生改善心肌梗死后小鼠心脏重构[J].中国病理生理杂志,2018,34(1):47-51,5.基金项目
国家卫生和计划生育委员会科研基金资助项目(No.WKJ-ZJ-1725) （No.WKJ-ZJ-1725）
