中国病理生理杂志2018，Vol.34Issue(1)：70-74,5.DOI:10.3969/j.issn.1000-4718.2018.01.012
川楝素对人卵巢癌细胞侵袭和迁移的影响
Effect of toosendanin on invasion and migration of human ovarian cancer cells
摘要
Abstract
AIM:To investigate the effect of toosendanin(TSN)on invasion and migration abilities of human ovarian cancer cells and the related mechanism.METHODS:The human ovarian cancer cell lines CAVO-3 and SKVO-3 were treated with TSN at different concentrations.The cell viabilty at 12,24,48,72 and 96 h after TSN treatment was measured by CCK-8 assay.Scratch wound healing assay and Transwell assay were employed to measure the invasion and migration abilities of CAVO-3 cells.The protein expression of nuclear factor-κB(NF-κB)p65,E-cadherin,N-cadherin,vimentin and Snail was determined by Western blot.RESULTS:TSN significantly inhibited the viability of CAVO-3 and SKVO-3 cells(P<0.05 ).Compared with control group ,the migration and invasion abilities of CAVO-3 cells in TSN group decreased significantly(P<0.05).In addition,the expression of NF-κB p65 and E-cadherin protein increased no-tably,followed with N-cadherin,vimentin and Snail protein decreased significantly(P<0.05).However,the inhibitor of NF-κB BAY11-7082 reversed the impact above.Compared with TSN group ,the migration and invasion abilities in TSN +BAY11-7082 group increased significantly(P<0.05).The protein expression of E-cadherin also decreased notably ,fol-lowed with the protein expression of N-cadherin,vimentin and Snail increased significantly(P<0.05).CONCLUSION:TSN inhibits the invasion and migration abilities of human ovarian cancer cells ,which is related to the inhibition of epitheli-al-mesenchymal transition process mediated by NF-κB/Snail signaling pathway.关键词
川楝素/卵巢癌/细胞迁移/细胞侵袭/上皮-间充质转化/核因子κBKey words
Toosendanin/Ovarian cancer/Cell invasion/Cell migration/Epithelial-mesenchymal transition/Nuclear factor-κB分类
医药卫生引用本文复制引用
李雨颖,章科娜,蔡锦威,林江涛,邵喜英..川楝素对人卵巢癌细胞侵袭和迁移的影响[J].中国病理生理杂志,2018,34(1):70-74,5.基金项目
衢州市科技计划(No.2016Y018) （No.2016Y018）
衢州市重点创新团队经费资助项目 （）
