中国病理生理杂志2018，Vol.34Issue(1)：118-122,5.DOI:10.3969/j.issn.1000-4718.2018.01.020
外源性硫化氢对肝细胞NLRP3炎症小体的影响
Effect of exogenous hydrogen sulfide on expression of NLRP3 inflamma-some in hepatocytes
摘要
Abstract
AIM:To evaluate the effect of exogenous hydrogen sulfide(H2S)on the expression of NLRP3 in-flammasome in hepatocytes.METHODS:The hepatocytes L 02 and SMMC-7721 were used to establish the model of inflam-mation by stimulating with lipopolysaccharide(LPS)at different concentrations in vitro.The expression of NLRP3 inflam-masome in the hepatocytes was detected by Western blot and the cell viability was measured by MTT assay for determining appropriate concentration of LPS.The hepatocytes were divided into 4 groups:the cells in control group were incubated with normal medium for 18.5 h;the cells in LPS group were incubated with normal medium for 0.5 h followed by 100 μg/L LPS for 18 h;the cells in LPS+H2 S group and H 2 S group were incubated with 200μmol/L sodium hydrosulfide hydrate(NaHS)for 0.5 h followed by 100 μg/L LPS or normal medium for 18 h,respectively.The protein expression of NLRP3 and caspase-1 in the cells of every group was determined by Western blot.RESULTS:Compared with control group ,the protein expression of NLRP3 and caspase-1 increased significantly in LPS group(P<0.05)and had no significant change in H2S group.Compared with LPS group,the protein expression of NLRP3 and caspase-1 in LPS+H2S group decreased significantly(P<0.05).CONCLUSION:In hepatocytes,exogenous H2S suppresses the expression of NLRP3 inflamma-some.关键词
硫化氢/NLRP3炎症小体/肝细胞/脂多糖Key words
Hydrogen sulfide/NLRP3 inflammasome/Hepatocytes/Lipopolysaccharide分类
医药卫生引用本文复制引用
王红钢,李彦章,孙伟力,钟培育,吴东栋,王军,蔡欢,王国英,乔玲,张涛..外源性硫化氢对肝细胞NLRP3炎症小体的影响[J].中国病理生理杂志,2018,34(1):118-122,5.基金项目
国家自然科学基金资助项目(No.31300841) （No.31300841）
河南省高校重点科研项目(No.16A310001) （No.16A310001）
河南大学2016年度大学生创新性实验计划(No.16NB047) （No.16NB047）
