中国病理生理杂志2018，Vol.34Issue(1)：141-146,6.DOI:10.3969/j.issn.1000-4718.2018.01.024
转录因子MAZ对c1orf109基因转录表达调控的体外研究
Regulation of c1orf109 gene by transcription factor MAZ in vitro
摘要
Abstract
AIM:To study the regulation of human chromosome 1 open reading frame 109(c1orf109)gene by transcription factor Myc-associated zinc-finger protein(MAZ)in vitro.METHODS:In vitro study,electrophoretic mobili-ty shift assay was performed to screen the binding sites of MAZ in the promoter region of c1orf109 gene.The HeLa cells were co-transfected with the enhanced green fluorescent protein reporter vector driven by c1orf109 promoter,and MAZ and transcription factor specificity protein 1(Sp1)expression plasmids.After 24 h,the transcriptional expression of c1orf109 gene in the co-transfected cells was determined by confocal scanning microscopy and flow cytometry.RESULTS:The c1orf109 promoter could bind to MAZ ,and shared the binding sites with Sp 1.MAZ and Sp1 both inhibited the transcrip-tional expression of c1orf109 gene and the inhibition effect of Sp1 was greater than MAZ(P<0.05).CONCLUSION:Both MAZ and Sp1 regulate the expression of c1orf109 gene in physiological and pathological conditions ,and this regulation is redundancy with the same direction.The existence of redundancy transcriptional regulation manner of this gene suggests that precise regulation of c1orf109 gene is vital important for cell biological processing.关键词
转录因子/Myc相关锌指蛋白/特化蛋白1/c1orf109基因Key words
Transcription factors/Myc-associated zinc-finger protein/Specificity protein 1/c1orf109 gene分类
医药卫生引用本文复制引用
王雪伟,任剑波,王小怡,王博,高哲,郭大玮..转录因子MAZ对c1orf109基因转录表达调控的体外研究[J].中国病理生理杂志,2018,34(1):141-146,6.基金项目
国家自然科学基金资助项目(No.90608008 ) （No.90608008 ）
高等学校博士学科点专项科研基金博导类资助项目(No.20111417110003) （No.20111417110003）
