中国动脉硬化杂志2017，Vol.25Issue(12)：1189-1195,7.
钙离子信号蛋白TRPC1及Orai1参与了HUVEC经SOC和ROC介导的钙内流和NO生成
Calcium signaling proteins TRPC1 and Orai1 participate in Ca2+ entry and NO generation mediated by SOC and ROC in human umbilical vein endothelial cell
摘要
Abstract
Aim To study the function of TRPC1 and Orai1 in store and receptor-operated Ca2+ entry and nitric oxide generation by SOC and ROC in human umbilical vein endothelial cell.Methods HUVEC were collected and cultured to the second-third passage.We silenced the expression of their genes in HUVEC by transfection constructed TR-PC1 or Orai1 RNA interference plasmids.The interference efficiency of their proteins and mRNA levels were determined by Western blot and real-time PCR,respectively.The cell were incubated with CaR agonist spermine,CaR negative allosteric modulator Calhex231 and ROC analogue TPA,protein kinase C (PKC) inhibitor Ro31-8220,PKCs and PKCμ inhibitor Go6967.Intracellular Ca2+ concentration ([Ca2+] i) was detected using the fluorescence Ca2+ indicator Fura-2/ AM,the production of NO was determined by DAF-FM of every group in HUVEC.Results Compared with control group,shRNA targeted to the TRPCI or Orai1 genes decreased their mRNA and protein levels,respectively (P＜0.05);The results of their mRNA levels by 84.50％ and 76.10％ and proteins levels were decreased by 83.98％ and 71.73％;In four different treatment under the action of factors,the [Ca2+] i and the net NO fluorescence intensity ratio values of transfection of TRPC1shRNA and Orai1shRNA group were significantly reduced (P＜0.05).Conclusion TRPC1 and Orail participated in CaR-mediated Ca2+ influx and NO production activation mediated by SOC and ROC in HUVEC.关键词
瞬时受体电位通道1/钙释放激活钙通道调节分子1/钙敏感受体/钙离子/一氧化氮Key words
Transient receptor potential canonical 1/Calcium release-activated calcium modulator 1/Ca2+-sensing receptor/Ca2+/Nitric oxide分类
医药卫生引用本文复制引用
王腊梅,钟华,唐娜,庞丽娟,孙志萍,何芳..钙离子信号蛋白TRPC1及Orai1参与了HUVEC经SOC和ROC介导的钙内流和NO生成[J].中国动脉硬化杂志,2017,25(12):1189-1195,7.基金项目
国家自然科学基金资助项目(31160239 （）
81160018) （）
