中国动脉硬化杂志2017，Vol.25Issue(12)：1207-1211,5.
长期高盐所致高血压大鼠主动脉血管平滑肌细胞大电导钙激活钾通道电流的变化
Changes of large-conductance calcium-activated potassium channels in vascular smooth muscle cells in rats of hypertension induced by long-term high-salt diet
摘要
Abstract
Aim To observe the changes of blood pressure level and large-conductance calcium-activated potassium channels (BKCa) current in aortic vascular smooth muscle cell (VSMC) in rats after high-salt diet.Methods 3 weeks old male Wister rats were randomly divided into two groups:control group and model group,24 rats in each group.The control group was given with normal diet (containing 0.5％ NaCl),and the model group was treated with high-salt diet (containing 4％ NaC1).The blood pressure in rats was measured by tail artery manometry.Whole-cell patch clamp technique was used for recording BKCa currents in rat aortic VSMC membrane.Results In the model group,the blood pressure increased significantly from the eighth week,and the blood pressure gradually increased with the prolongation of the high-salt diet time.At the tenth,twelfth,fourteenth and sixteenth week,the blood pressure in the model group was significantly higher than that in the control group (P＜0.01).The BKCa current and current density of aortic VSMC membrane in the model group increased significantly with the prolongation of the high-salt diet time.At the eighth,twelfth and sixteenth week,the peak current density of BKCa in the model group was significantly higher than that in the control group,and the difference was statistically significant (P＜0.05).Conclusion High-salt diet can lead to elevated blood pressure in rats.The BKCa current density in rats increases gradually with the increase of blood pressure due to high-salt diet.关键词
高盐饮食/高血压/血管平滑肌细胞/大电导钙激活钾通道Key words
High-salt diet/Hypertension/Vascular smooth muscle cell/Large-conductance calcium-activated potassium channels分类
医药卫生引用本文复制引用
王云霞,吴志婷,石秀明,盛国太,葛郁芝..长期高盐所致高血压大鼠主动脉血管平滑肌细胞大电导钙激活钾通道电流的变化[J].中国动脉硬化杂志,2017,25(12):1207-1211,5.基金项目
国家自然科学基金资助项目(81260031) （81260031）
江西省卫生计生委科技计划项目(20155056、20161002) （20155056、20161002）
江西省科技厅科研院所基础设施配套项目(20142BBA13042) （20142BBA13042）
