中国动脉硬化杂志2017，Vol.25Issue(12)：1212-1218,7.
甘草查尔酮A对THP-1巨噬细胞促炎因子表达的影响——依赖于TLR-4/NF-κB炎症信号通路
Effect of licochalcone A on expression of proinflammatory cytokines in THP-1 macrophages——Dependent on the TLR-4/NF-κB inflammatory signaling pathway
摘要
Abstract
Aim To investigate the effects of licochalcone A (Lico A)on the expression of inflammatory cytokines in macrophages of human acute monocytic leukemia cell line (THP-1) induced by lipopolysaccharide (LPS) in vitro.Methods 100 μg/L phorbol ester (PMA) was used to induce THP-1 cells for 48 hours,after the differentiation of THP-1 cells,which became macrophage,and cells were divided into control group,LPS group and LPS combined with concentration (20,10,5 mg/L) of Lico A.The levels of interleukin-1 beta (IL-1β),interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) were detected by ELISA.The levels of Toll like receptor-4 (TLR-4) and nuclear factor kappa B (NF-κB) mRNAs were tested by real-time PCR.The levels of TLR-4,NF-κB,I kappa B kinase alpha (IKKα),phosphorylated IKB-alpha (p-IKB-α),cyclooxygenase-2 (COX-2) and isoform of nitric oxide synthase (iNOS) proteins were examined by Western blot.Results The expression levels of TNF-α,IL-1 and IL-6 were up-regulated in the v macrophages after stimulated by LPS,Lico A could reduce the elevated expression levels of TNF-α,IL-1 and IL-6 induced by LPS.The expression of TLR-4 significantly increased after stimulated by LPS and NF-κB was activated.Lico A could reverse the above changes and prevent the activation of NF-κB.Conclusion Lico A could inhibit LPS-induced inflammatory response in THP-1 macrophages via TLR-4/NF-κB pathway.关键词
甘草查尔酮A/THP-1细胞/Toll样受体4/核因子κBKey words
Licochalcone A/THP-1 cells/Toll-like receptor-4/Nuclear factor kappa B分类
医药卫生引用本文复制引用
龙小琴,戴应和,袁经权,易蔚..甘草查尔酮A对THP-1巨噬细胞促炎因子表达的影响——依赖于TLR-4/NF-κB炎症信号通路[J].中国动脉硬化杂志,2017,25(12):1212-1218,7.基金项目
广西中药药效研究重点实验室开放项目(14-045-12) （14-045-12）
