中国动脉硬化杂志2017，Vol.25Issue(12)：1219-1224,6.
7-二氟亚甲基-5,4'-二甲氧基染料木黄酮通过下调Caspase-3的表达拮抗H2O2诱导的血管内皮细胞凋亡
The anti-apoptotic effects of 7-difluoromethyl-5,4'-dimethoxygenistein on vascular endothelial cells induced by H2O2 through down-regulating the expression of Caspase-3
摘要
Abstract
Aim To study the effects of 7-difluoromethyl-5,4'-dimethoxygenistein (dFMGEN) on the apoptosis to H2O2-induced vascular endothelial cells and its molecular mechanism.Methods The oxidative stress injury model was established by human umbilical vein endothelial cells induced by 1 mmol/L H2O2.The experiment was divided into blank control group,H2O2 group,vehicle group,100 μmol/L genistein group and different concentrations (0.1,0.3,1,3,10 μ mol/L) dFMGEN groups.The reactive oxygen species (ROS) generation was examined by DCFH-DA activated fluorescence flow cytometry (FCM).Apoptotic morphology was observed by acridine orange staining under fluorescence microscopy.The apoptotic rate was detected by PI staining FCM.The expression of Caspase-3 was determined by Western blot.Results When vascular endothelial cells were treated with 1 mmol/L H2O2 for 24 h,the generation of ROS was highly added,obvious morphological changes of apoptosis was found by acridine orange staining under fluorescence microscopy,the apoptotic rate was increased and the expression of Caspase-3 was up-regulated.After pretreating with dFMGEN,results showed that dFMGEN could reduce the release of ROS,decrease the apoptotic rate and down-regulate the expression of Caspase-3.Conclusion dFMGEN can effectually inhibit the apoptosis of vascular endothelial cells induced by H2O2,which is probably due to the decrease of ROS generation and downregulation of Caspase-3,thereby inhibiting apoptosis of vascular endothelial cells induced by H2O2.关键词
7-二氟亚甲基-5,4'-二甲氧基染料木黄酮/Caspase-3/过氧化氢/细胞凋亡Key words
7-difluoromethyl-5,4'-dimethoxygenistein/Caspase-3/H2O2/Apoptosis分类
医药卫生引用本文复制引用
王莉,李素云,彭田红,何慧,吕运成,符晓华,曹建国..7-二氟亚甲基-5,4'-二甲氧基染料木黄酮通过下调Caspase-3的表达拮抗H2O2诱导的血管内皮细胞凋亡[J].中国动脉硬化杂志,2017,25(12):1219-1224,6.基金项目
湖南省教育厅科研资助项目(10C1178) （10C1178）
