中国动脉硬化杂志2017，Vol.25Issue(12)：1225-1230,6.
镁离子对高磷诱导的大鼠胸主动脉血管环钙化的影响
The effects of magnesium ions on the calcification of aortic rings induced by high phosphorus in rats
摘要
Abstract
Aim To investigate the effect and possible mechanisms of the different concentrations of magnesium ions on aortic rings calcification induced by high phosphorus in rats.Methods Aortic rings were isolated from rat thoracic aorta and cultured in vitro,and randomly divided into control group,high phosphorus group and magnesium intervention group(final concentrations of magnesium ions were 1,2 and 3 mmol/L).Aortic rings were cultured with 10％ fetal bovine serum in control group.After 14 days of intervention,the expressions of L-type calcium channel (LTCC)α1c β3 subunit,Runt-related transcription factor 2 (Runx2) and smooth muscle 22α (SM22α) were detected by Immunohistochemistry.Calcium concentration of aortic rings was measured by Von Kossa staining and quantification of calcium.Results Compared with control group,calcified nodules in high phosphorus group was increased,and calcified nodules gradually reduced with the increased magnesium ion concentration in the intervention group.The expression of LTCCα1c、β3 subunits in high phosphorus group increased (P＜0.05).With the increase of magnesium ion concentration,the expression of Runx2、LTCC α1c、β3 decreased (P＜0.05) and the expression of SM22α increased (P＜0.05) in high magnesium group.There results of Pearson correlation analysis showed that SM22α was negatively corelated with Runx2 (r =-0.671,P=0.001),and LTCCα1c subunit was positively corelated with Runx2(r=0.712,P＜0.001),LTCCβ3 subunit was positively corelated with Runx2(r=0.0.692,P＜0.001).Conclusion Magnesium can promote high phosphorus induced rat aortic rings calcification,and its mechanism is possibly achieved by downregulatiing LTCC α1c β3 protein expression,and reducing the transformation of VSMCs into osteogenic/osteogenic phenotype.关键词
镁离子/血管环/钙化/表型转化/L型钙通道Key words
Magnesium/Aortic rings/Vascular calcification/Osteogenic differentiation/L-type calcium channel分类
医药卫生引用本文复制引用
张慧然,徐金升,袁景霞,白亚玲,张俊霞,张胜雷..镁离子对高磷诱导的大鼠胸主动脉血管环钙化的影响[J].中国动脉硬化杂志,2017,25(12):1225-1230,6.基金项目
河北省卫计委计划项目(20150351 （）
20150310) （）
河北省科技计划项目(16397733D) （16397733D）
