中国水稻科学2018，Vol.32Issue(1)：12-22,11.DOI:10.16819/j.1001-7216.2018.7055
水稻OsENO2-2基因过表达对水稻抽穗期的影响
Overexpression of OsENO2-2 Affects Heading Date in Rice
摘要
Abstract
[Objective]OsENO2-2 was isolated from japonica cultivar Zhonghua 11 as a short transcript produced by alternative splicing of OsENO2 which is homologous to AtENO2. To reveal the function of OsENO2-2,[Method]we constructed an overexpression vector of OsENO2-2 and obtained the transgenetic plants. Based on the observation and statistics of phenotype in overexpressed plant and wild-type, we validated the function of OsENO2-2 using reverse genetics method. [Result]Overexpression of OsENO2-2 transgenetic plants delayed heading date only under long-day conditions but not short-day conditions. qRT-PCR expression analysis of the critical flowering time genes showed that in long-day conditions, the expression of RFT1 in overexpressed plants was downregulated, and the expression levels of the other tested genes did not change significantly in wild-type and overexpressed plants; but in short-day conditions, the expression of all the tested genes in wild-type and over-expression plants did not change significantly. [Conclusion]OsENO2-2 might be involved in the photoperiodic pathways to regulate the heading date in rice by regulating the expression of RFT1 gene.关键词
水稻/过量表达/OsENO2-2/烯醇化酶基因/抽穗期Key words
rice/overexpression/OsENO2-2/enolase gene/heading date分类
生物科学引用本文复制引用
刘小云,李晓,李腾飞,苏鲁方..水稻OsENO2-2基因过表达对水稻抽穗期的影响[J].中国水稻科学,2018,32(1):12-22,11.基金项目
国家自然科学基金资助项目(31600981) （31600981）
江汉大学科研启动项目(1019-06210002). （1019-06210002）
