中国水稻科学2018，Vol.32Issue(1)：23-34,12.DOI:10.16819/j.1001-7216.2018.7075
水稻耐金属离子胁迫的QTL分析
Identifying of QTLs for Resistance to Metal Irons Stress in Rice
摘要
Abstract
[Objective]The molecular markers linked to the tolerance of various metal ions during rice seeding stage were screened for the purpose of discussing the genetic basis of the resistance to different metal ions.[Method]A rice double haploid(DH) population, derived from a typical indica-japonica cross(Chunjiang 06/TN1) via the anther culture was used to analyze the resistance of four metal ions(Fe2+, Cd2+, Al3+, Na+) in DH population and its parents, then the quantitative trait loci (QTL) for the resistance of the four metal ions were identified using an available and complete molecular linkage map. In addition, real-time quantitative PCR was used to detect the expression of related genes before and after treatment.[Result]A total of 8 QTLs for resistance to metal ions stress were detected, which were localized on chromosomes 1, 2, 4, 6, 9, 10 and 11. Among them, one QTL with the greatest contribution for resistance to Fe2+treatment(24.47%, threshold is 7.78) located in the region RM1297-RM1061 on chromosome 1. Meanwhile, the expression analysis of the genes related to stress tolerance in this interval showed that these genes had different expression levels before and after treatment. One QTL was detected after Cd2+ treatment, which was located on chromosome 1. Five QTLs were detected after Al3+ treatment, which were located on chromosomes 2, 4, 6, 10 and 11 respectively. And one QTL was detected after Na+ treatment, which was located on chromosome 9. [Conclusion]According to the QTL analysis of phenotypic differences among DH groups which were treated with different metal ions, eight QTLs for resistance to various metal ions were detected. It laid the foundation for the fine mapping and cloning of the corresponding QTL and exploring the QTL molecular regulation mechanism of the resistance to different metal ions in rice.关键词
水稻/抗性/金属胁迫/QTL分析Key words
rice/resistance/metal ion stress/QTL analysis分类
生物科学引用本文复制引用
林晗,徐江民,胡瑚倩,郑安,徐婉璐,漏平,王跃星,曾大力,饶玉春..水稻耐金属离子胁迫的QTL分析[J].中国水稻科学,2018,32(1):23-34,12.基金项目
国家转基因生物新品种培育重大科技专项(2016ZX08009003-003-008) （2016ZX08009003-003-008）
浙江省自然科学基金资助项目(LY16C130001) （LY16C130001）
浙江省科协育才工程资助项目(2017YCGC008) （2017YCGC008）
水稻生物学国家重点实验室开放资助项目. （）
