中国水稻科学2018，Vol.32Issue(1)：43-50,8.DOI:10.16819/j.1001-7216.2018.7011
应用东乡野生稻回交重组自交系群体分析糙米矿质含量QTL
QTLAnalysis for Mineral Contents in Brown Rice Using a BC2F4:5 Population Derived from Dongxiang Wild Rice (Oryza rufipogon Griff.)
摘要
Abstract
[Objective]Biofortifying food crops with essential minerals would alleviate mineral deficiencies in humans.[Method]One plant A58 was selected from Xieqingzao B// Xieqingzao B /Dongxiang wild rice BC1F5 population, and was backcrossed with Xieqingzao B to develop a BC2F4:5 population. The contents of Mg, Ca, Zn, Fe, Mn and Cu in brown rice of 132 BC2F4:5 lines were measured with an inductively coupled plasma atomic emission spectrometer (ICP-AES). Detection of quantitative trait loci (QTLs) for mineral contents in brown rice was conducted using Windows QTL Cartographer 2.5.[Result]A total of 17 QTLs for mineral contents in brown rice were detected on chromosomes 1, 4, 6, 8, 9 and 11, respectively, including one for Mg content, four for Ca content, four for Zn content, two for Fe content, two for Mn content, and four for Cu content. The explained phenotypic variations ranged from 5.0% to 47.2%, and eight QTLs of them had the enhancing alleles derived from Oryza rufipogon. [Conclusion]Twelve QTLs were clustered in five chromosomal regions, indicating that common genetic-physiological mechanisms were involved for different mineral nutrients, and the beneficial alleles could be utilized to improve grain nutritional quality by marker-assisted selection.关键词
东乡野生稻/糙米/矿质含量/QTL定位Key words
Dongxiang wild rice/brown rice/mineral content/QTL mapping分类
生物科学引用本文复制引用
胡标林,黄得润,肖叶青,何强生,万勇,樊叶杨..应用东乡野生稻回交重组自交系群体分析糙米矿质含量QTL[J].中国水稻科学,2018,32(1):43-50,8.基金项目
国家863计划资助项目(2014AA10A604) （2014AA10A604）
江西省重点研发计划重大专项(20161ACF60022) （20161ACF60022）
江西省自然科学基金资助项目(20171ACB21071) （20171ACB21071）
江西省农业科学院创新基金资助项目(20161CBS002). （20161CBS002）
