中国药业2018，Vol.27Issue(1)：9-13,5.DOI:10.3969/j.issn.1006-4931.2018.01.003
柴胡皂苷D对骨肉瘤143B细胞增殖抑制作用研究
Inhibitory Effect of Saikosaponin D on Proliferation of Osteosarcoma 143B Cells
摘要
Abstract
Objective To investigate the effects of saikosaponin D(SS - d) on the growth of osteosarcoma 143B cells, and to explore its mechanism preliminarily. Methods The proliferation of osteosarcoma 143B cells was detected by MTT assay at 6, 12, 24, 48, 72 h after treated with different concentrations of SS - d. The expression of human osteocalcin(OCN) was detected by ELISA, and the alkaline phosphatase(AKP) activity was detected by AKP test cassette. Results Low concentrations of SS - d could significantly inhibite the growth of osteosarcoma 143B cells in a dose - dependent manner( P < 0. 05) . The AKP activity test showed that there was no signifi-cant change in AKP activity in 143B cells treated with saikosaponin. ELISA result showed that saikosaponin could attenuate the expression of OCN. Conclusion SS - d can inhibit the proliferation of 143B cells, and the activity is the strongest, its mechanism may be unrelated to the promotion of osteogenic differentiation.关键词
骨肉瘤/柴胡皂苷D/碱性磷酸酶/骨钙素/肿瘤细胞/细胞增殖Key words
osteosarcoma/saikosaponin D/alkaline phosphatase/osteocalcin/tumor cell/cell proliferation分类
医药卫生引用本文复制引用
杨春艳,范文翔,欧学兰,袁斌..柴胡皂苷D对骨肉瘤143B细胞增殖抑制作用研究[J].中国药业,2018,27(1):9-13,5.基金项目
四川省教育厅资助项目[15ZB0190] （）
四川省南充市科技局项目[14A0064]. （）
